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Prion protein library of recombinant constructs for structural biology

Academic Article
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Overview

authors

  • Hornemann, S.
  • Christen, B.
  • von Schroetter, C.
  • Perez, D. R.
  • Wuthrich, Kurt

publication date

  • April 2009

journal

  • FEBS Journal  Journal

abstract

  • A survey of plasmids for 51 prion protein constructs from bank vole, cat, cattle, chicken, dog, elk, ferret, frog, fugu, horse, human, pig, sheep, turtle, and wallaby, and for 113 mouse prion protein constructs and variants thereof, is presented. This includes information on the biochemistry of the recombinant proteins, in particular on successful and unsuccessful expression attempts. The plasmid library was generated during the past 12 years in the context of NMR structure determination and biophysical characterization of prion proteins in our laboratory. The plasmids are now available for general use, and are distributed free of charge to not-for-profit institutions.

subject areas

  • Animals
  • Cats
  • Cattle
  • Dogs
  • Gene Library
  • Humans
  • Magnetic Resonance Spectroscopy
  • Mice
  • Prions
  • Recombinant Proteins
  • Sheep
  • Swine
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Research

keywords

  • NMR structure determination
  • prion protein plasmid library
  • prion protein structural biology
  • recombinant prion proteins
  • transmissible spongiform encephalopathies
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Identity

International Standard Serial Number (ISSN)

  • 1742-464X

Digital Object Identifier (DOI)

  • 10.1111/j.1742-4658.2009.06968.x

PubMed ID

  • 19348007
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Additional Document Info

start page

  • 2359

end page

  • 2367

volume

  • 276

issue

  • 8

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