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The bacillus subtilis response regulator spooa stimulates transcription of the spoiig operon through modification of rna polymerase promoter complexes

Academic Article
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Overview

authors

  • Bird, T. H.
  • Grimsley, J. K.
  • Hoch, James
  • Spiegelman, G. B.

publication date

  • March 1996

journal

  • Journal of Molecular Biology  Journal

abstract

  • Sporulation in Bacillus subtilis is dependent on the response regulator Spo0A, which both represses and activates transcription in vitro. The activity of Spo0A is increased by phosphorylation. We previously demonstrated that the phosphorylation increased the ability of Spo0A to stimulate in vivo transcription from the promoter for the spoIIG operon, one of the operons known to be regulated by Spo0A in vivo. In the work reported here we have examined the kinetics of transcription initiation at the spoIIG operon promoter using a single round transcription assay and the kinetics of formation of spoIIG promoter-RNA polymerase complexes using DNase I footprinting. Both the kinetic assays and the footprint assays indicated that the initial binding of the polymerase to the template was not dependent on the presence of Spo0A. The phosphorylated form of Spo0A stimulated the rate of initiation by affecting a step that occurred after the initial interaction of the polymerase with the template. Phosphorylation of Spo0A may stimulate transcription by modifying preinitiation complexes containing the polymerase and the promoter.

subject areas

  • Bacillus subtilis
  • Bacterial Proteins
  • Base Sequence
  • DNA Footprinting
  • DNA, Bacterial
  • DNA-Directed RNA Polymerases
  • Deoxyribonuclease I
  • Enzyme Inhibitors
  • Gene Expression Regulation, Bacterial
  • Heparin
  • Kinetics
  • Molecular Sequence Data
  • Nucleotides
  • Operon
  • Phosphorylation
  • Promoter Regions, Genetic
  • Sigma Factor
  • Spores, Bacterial
  • Transcription Factors
  • Transcription, Genetic
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Research

keywords

  • Bacillus subtilis
  • SpoOA
  • response regulators
  • sporulation
  • transcription activation
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Identity

International Standard Serial Number (ISSN)

  • 0022-2836

Digital Object Identifier (DOI)

  • 10.1006/jmbi.1996.0099

PubMed ID

  • 8604129
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Additional Document Info

start page

  • 436

end page

  • 448

volume

  • 256

issue

  • 3

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