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A simple method for detecting up to five immunofluorescent parameters together with DNA staining for cell cycle or viability on a benchtop flow cytometer

Academic Article
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Overview

authors

  • Gordon, K. M.
  • Duckett, L.
  • Daul, B.
  • Petrie, Howard

publication date

  • April 2003

journal

  • Journal of Immunological Methods  Journal

abstract

  • In this manuscript, we describe modifications to a commercial three-laser benchtop flow cytometer, as well as relevant biological methods, that allow analysis of up to five immunofluorescent parameters together with an ultraviolet (UV)-excitable DNA stain. This method allows expanded capacity for multiparameter immunophenotyping of complex mixed cell populations, together with accurate measurements of DNA content (cell cycle) or cell viability, on a stable, end-user operated platform.

subject areas

  • Cell Cycle
  • Cell Survival
  • DNA
  • Flow Cytometry
  • Fluorescent Antibody Technique
  • Fluorescent Dyes
  • Indoles
  • Lasers
  • Propidium
  • Staining and Labeling
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Research

keywords

  • DNA staining
  • cell cycle analysis
  • flow cytometry
  • immunofluorescence
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Identity

International Standard Serial Number (ISSN)

  • 0022-1759

Digital Object Identifier (DOI)

  • 10.1016/s0022-1759(03)00009-7

PubMed ID

  • 12667675
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Additional Document Info

start page

  • 113

end page

  • 121

volume

  • 275

issue

  • 1-2

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