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Global identification of caspase substrates using PROTOMAP (Protein Topography and Migration Analysis Platform)

Academic Article
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Overview

authors

  • Dix, Melissa
  • Simon, Gabriel
  • Cravatt, Benjamin

publication date

  • 2014

journal

  • Methods in Molecular Biology  Journal

abstract

  • Delineation of the natural substrate scope of proteases is important for understanding the functions of proteolytic pathways in physiology and disease. Herein we describe the protocol for PROTOMAP, a technique that combines SDS-PAGE with tandem mass spectrometry to globally identify shifts in protein migration indicative of proteolytic processing. When applied to cells undergoing apoptosis, this unbiased global method provides a snapshot of the topography and magnitude of proteolytic events associated with programmed cell death.

subject areas

  • Apoptosis
  • Caspases
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Molecular Biology
  • Proteomics
  • Substrate Specificity
  • Tandem Mass Spectrometry
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Identity

International Standard Serial Number (ISSN)

  • 1940-6029 (Electronic) 1064-3745 (Linking)

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-0357-3_3

PubMed ID

  • 24567094
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Additional Document Info

start page

  • 61

end page

  • 70

volume

  • 1133

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