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Restraint stress alters nociceptin/orphanin FQ and CRF systems in the rat central amygdala: significance for anxiety-like behaviors

Academic Article
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Overview

authors

  • Ciccocioppo, R.
  • de Guglielmo, G.
  • Hansson, A. C.
  • Ubaldi, M.
  • Kallupi, M.
  • Cruz, M. T.
  • Oleata, C. S.
  • Heilig, M.
  • Roberto, Marisa

publication date

  • January 2014

journal

  • Journal of Neuroscience  Journal

abstract

  • Corticotropin releasing factor (CRF) is the primary mediator of stress responses, and nociceptin/orphanin FQ (N/OFQ) plays an important role in the modulation of these stress responses. Thus, in this multidisciplinary study, we explored the relationship between the N/OFQ and the CRF systems in response to stress. Using in situ hybridization (ISH), we assessed the effect of body restraint stress on the gene expression of CRF and N/OFQ-related genes in various subdivisions of the amygdala, a critical brain structure involved in the modulation of stress response and anxiety-like behaviors. We found a selective upregulation of the NOP and downregulation of the CRF1 receptor transcripts in the CeA and in the BLA after body restraint. Thus, we performed intracellular electrophysiological recordings of GABAA-mediated IPSPs in the central nucleus of the amygdala (CeA) to explore functional interactions between CRF and N/OFQ systems in this brain region. Acute application of CRF significantly increased IPSPs in the CeA, and this enhancement was blocked by N/OFQ. Importantly, in stress-restraint rats, baseline CeA GABAergic responses were elevated and N/OFQ exerted a larger inhibition of IPSPs compared with unrestraint rats. The NOP antagonist [Nphe1]-nociceptin(1-13)NH2 increased the IPSP amplitudes in restraint rats but not in unrestraint rats, suggesting a functional recruitment of the N/OFQ system after acute stress. Finally, we evaluated the anxiety-like response in rats subjected to restraint stress and nonrestraint rats after N/OFQ microinjection into the CeA. Intra-CeA injections of N/OFQ significantly and selectively reduced anxiety-like behavior in restraint rats in the elevated plus maze. These combined results demonstrate that acute stress increases N/OFQ systems in the CeA and that N/OFQ has antistress properties.

subject areas

  • Amygdala
  • Animals
  • Anxiety
  • Behavior, Animal
  • Corticotropin-Releasing Hormone
  • Disease Models, Animal
  • In Situ Hybridization
  • Male
  • Opioid Peptides
  • Patch-Clamp Techniques
  • Rats
  • Rats, Wistar
  • Restraint, Physical
  • Stress, Psychological
  • Transcriptome
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Identity

PubMed Central ID

  • PMC3870926

International Standard Serial Number (ISSN)

  • 0270-6474

Digital Object Identifier (DOI)

  • 10.1523/jneurosci.2400-13.2014

PubMed ID

  • 24403138
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Additional Document Info

start page

  • 363

end page

  • 372

volume

  • 34

issue

  • 2

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