Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Liver-directed gene transfer: a linear polyethylenimine derivative mediates highly efficient DNA delivery to primary hepatocytes in vitro and in vivo

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Chemin, I.
  • Moradpour, D.
  • Wieland, Stefan
  • Offensperger, W. B.
  • Walter, E.
  • Behr, J. P.
  • Blum, H. E.

publication date

  • November 1998

journal

  • Journal of Viral Hepatitis  Journal

abstract

  • Efficient DNA delivery is a prerequisite for the successful implementation of molecular antiviral strategies against chronic viral hepatitis and gene therapy in general. The cationic polymer polyethylenimine (PEI) has recently been explored as a gene transfer vector in various cell types in vitro and in vivo. In this study, we evaluated a linear PEI derivative (lPEI) as a vector for gene and oligodeoxynucleotide transfer into hepatocytes in vitro and in vivo. A simple protocol was developed that allowed transfection of up to 50% of primary hepatocytes in vitro. In addition, fluorescent oligodeoxynucleotides were efficiently delivered to the liver in vivo after intravenous injection into Pekin ducks. Thus, lPEI mediates highly efficient gene and oligodeoxynucleotide transfer into primary hepatocytes and is potentially useful for DNA delivery in vivo.

subject areas

  • Animals
  • Cells, Cultured
  • DNA
  • Ducks
  • Gene Transfer Techniques
  • Genetic Therapy
  • Genetic Vectors
  • Liver
  • Microscopy, Fluorescence
  • Oligodeoxyribonucleotides, Antisense
  • Polyethyleneimine
  • Transfection
scroll to property group menus

Research

keywords

  • Pekin duck
  • Tupaia belangeri
  • antisense oligodeoxynucleotide
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 1352-0504

Digital Object Identifier (DOI)

  • 10.1046/j.1365-2893.1998.00126.x

PubMed ID

  • 9857346
scroll to property group menus

Additional Document Info

start page

  • 369

end page

  • 375

volume

  • 5

issue

  • 6

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support