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An ensemble of theta class glutathione transferases with novel catalytic properties generated by stochastic recombination of fragments of two mammalian enzymes

Academic Article
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Overview

authors

  • Broo, K.
  • Larsson, A. K.
  • Jemth, P.
  • Mannervik, Bengt

publication date

  • April 2002

journal

  • Journal of Molecular Biology  Journal

subject areas

  • Animals
  • Catalysis
  • Clone Cells
  • Dinitrochlorobenzene
  • Epoxy Compounds
  • Evolution, Molecular
  • Gene Library
  • Glutathione Transferase
  • Humans
  • Isoenzymes
  • Kinetics
  • Methylene Chloride
  • Models, Molecular
  • Mutation
  • Naphthalenes
  • Nitrobenzenes
  • Nitrophenols
  • Rats
  • Recombinant Proteins
  • Sequence Analysis, DNA
  • Structure-Activity Relationship
  • Substrate Specificity
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Research

keywords

  • DNA shuffling
  • enzyme evolution
  • glutathione transferase
  • substrate specificity
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Identity

International Standard Serial Number (ISSN)

  • 0022-2836

Digital Object Identifier (DOI)

  • 10.1016/s00222836(02)00032-3

PubMed ID

  • 12054768
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Additional Document Info

start page

  • 59

end page

  • 70

volume

  • 318

issue

  • 1

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