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High-throughput screening technologies for botulinum neurotoxins

Academic Article
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Overview

authors

  • Bompiani, K. M.
  • Dickerson, Tobin

publication date

  • 2014

journal

  • Current Topics in Medicinal Chemistry  Journal

abstract

  • Botulinum neurotoxins (BoNTs) are a class of bacterial neurotoxins that are the most potent toxic compounds reported to date. Exposure to relatively low concentrations of the toxin protein can result in major muscle paralysis, which may result in death in severe cases. In addition to their role in natural human disease, BoNTs are currently under close scrutiny because of their potential to be used as biowarfare agents. Clinical treatment options for botulism are currently limited, and finite stockpiles of antitoxin exist. In light of current bioterrorist threats, researchers have focused on identifying new molecules that can be applied to either sensitive toxin detection or improved clinical treatment. High-throughput screening (HTS) is a laboratory technique commonly employed to screen large libraries of diverse compounds based on specific compound binding capabilities or function. Here we review existing HTS platforms that have been applied to identify novel BoNT diagnostic or therapeutic agents. HTS platforms for screening antibodies, peptides, small molecules, and aptamers are described, as well as the screening results and current progress of the identified compounds.

subject areas

  • Animals
  • Antibodies, Neutralizing
  • Antidotes
  • Aptamers, Nucleotide
  • Bioterrorism
  • Botulinum Toxins, Type A
  • Botulism
  • Chelating Agents
  • Enzyme Inhibitors
  • High-Throughput Screening Assays
  • Humans
  • Motor Neurons
  • Peptide Library
  • Peptidomimetics
  • Small Molecule Libraries
  • Synaptic Transmission
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Research

keywords

  • Antibody
  • antitoxin
  • aptamer
  • botulinum neurotoxin
  • botulism
  • high-throughput screening
  • small molecule
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Identity

International Standard Serial Number (ISSN)

  • 1568-0266

PubMed ID

  • 25335886
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Additional Document Info

start page

  • 2062

end page

  • 2080

volume

  • 14

issue

  • 18

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