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Small molecule targeting of a microRNA associated with hepatocellular carcinoma

Academic Article
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Overview

authors

  • Childs-Disney, J. L.
  • Disney, Matthew

publication date

  • February 2016

journal

  • ACS Chemical Biology  Journal

abstract

  • Development of precision therapeutics is of immense interest, particularly as applied to the treatment of cancer. By analyzing the preferred cellular RNA targets of small molecules, we discovered that 5"-azido neomycin B binds the Drosha processing site in the microRNA (miR)-525 precursor. MiR-525 confers invasive properties to hepatocellular carcinoma (HCC) cells. Although HCC is one of the most common cancers, treatment options are limited, making the disease often fatal. Herein, we find that addition of 5"-azido neomycin B and its FDA-approved precursor, neomycin B, to an HCC cell line selectively inhibits production of the mature miRNA, boosts a downstream protein, and inhibits invasion. Interestingly, neomycin B is a second-line agent for hepatic encephalopathy (HE) and bacterial infections due to cirrhosis. Our results provocatively suggest that neomycin B, or second-generation derivatives, may be dual functioning molecules to treat both HE and HCC. Collectively, these studies show that rational design approaches can be tailored to disease-associated RNAs to afford potential lead therapeutics.

subject areas

  • Antineoplastic Agents
  • Base Sequence
  • Carcinoma, Hepatocellular
  • DNA-Binding Proteins
  • Framycetin
  • Gene Expression Regulation, Neoplastic
  • Hep G2 Cells
  • Humans
  • Liver
  • Liver Neoplasms
  • MicroRNAs
  • Molecular Sequence Data
  • Molecular Targeted Therapy
  • Neoplasm Invasiveness
  • Transcription Factors
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Identity

PubMed Central ID

  • PMC4856042

International Standard Serial Number (ISSN)

  • 1554-8929

Digital Object Identifier (DOI)

  • 10.1021/acschembio.5b00615

PubMed ID

  • 26551630
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Additional Document Info

start page

  • 375

end page

  • 380

volume

  • 11

issue

  • 2

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