Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Ultrafast cooling reveals microsecond-scale biomolecular dynamics

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Polinkovsky, M. E.
  • Gambin, Y.
  • Banerjee, P. R.
  • Erickstad, M. J.
  • Groisman, A.
  • Deniz, Ashok

publication date

  • 2014

journal

  • Nature Communications  Journal

abstract

  • The temperature-jump technique, in which the sample is rapidly heated by a powerful laser pulse, has been widely used to probe the fast dynamics of folding of proteins and nucleic acids. However, the existing temperature-jump setups tend to involve sophisticated and expensive instrumentation, while providing only modest temperature changes of ~10-15 °C, and the temperature changes are only rapid for heating, but not cooling. Here we present a setup comprising a thermally conductive sapphire substrate with light-absorptive nano-coating, a microfluidic device and a rapidly switched moderate-power infrared laser with the laser beam focused on the nano-coating, enabling heating and cooling of aqueous solutions by ~50 °C on a 1-μs time scale. The setup is used to probe folding and unfolding dynamics of DNA hairpins after direct and inverse temperature jumps, revealing low-pass filter behaviour during periodic temperature variations.

subject areas

  • Cold Temperature
  • DNA
  • Hot Temperature
  • Inverted Repeat Sequences
  • Kinetics
  • Lasers
  • Light
  • Microfluidic Analytical Techniques
  • Molecular Dynamics Simulation
  • Nucleic Acid Conformation
  • Time Factors
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/ncomms6737

PubMed ID

  • 25517430
scroll to property group menus

Additional Document Info

volume

  • 5

©2019 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support