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The Caenorhabditis elegans unc-13 gene product is a phospholipid-dependent high-affinity phorbol ester receptor

Academic Article
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Overview

authors

  • Ahmed, S.
  • Maruyama, I. N.
  • Kozma, R.
  • Lee, J.
  • Brenner, Sydney
  • Lim, L.

publication date

  • November 1992

journal

  • Biochemical Journal  Journal

abstract

  • The Caenorhabditis elegans unc-13 mutant is a member of a class of mutants that have un-coordinated movement. Mutations of the unc-13 gene cause diverse defects in C. elegans, including abnormal neuronal connections and modified synaptic transmission in the nervous system. unc-13 cDNA encodes a protein (UNC-13) of 1734 amino acid residues with a predicted molecular mass of 198 kDa and sequence identity to the C1/C2 regions but not to the catalytic domain of the ubiquitously expressed protein kinase C family [Maruyama & Brenner (1991) Proc. Natl. Acad. Sci. U.S.A. 88, 5729-5733]. To characterize the phorbol ester binding site of the UNC-13 protein, cDNA encoding the C1/C2-like regions (amino acid residues 546-940) was expressed in Escherichia coli and the 43 kDa recombinant protein was purified. Phorbol ester binding to the 43 kDa protein was zinc- and phospholipid-dependent, stereospecific and of high affinity (Kd 67 nM). UNC-13 specific antisera detected a protein of approx. 190 kDa in wild-type (N2) but not in mutant (e1019) C. elegans cell extracts. We conclude that UNC-13 represents a novel class of phorbol ester receptor.

subject areas

  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Caenorhabditis elegans
  • Caenorhabditis elegans Proteins
  • Carrier Proteins
  • Cloning, Molecular
  • Consensus Sequence
  • Escherichia coli
  • Molecular Sequence Data
  • Peptide Fragments
  • Phorbol Esters
  • Phospholipids
  • Protein Kinase C
  • Receptors, Drug
  • Sequence Homology, Amino Acid
  • Zinc
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Identity

PubMed Central ID

  • PMC1133105

International Standard Serial Number (ISSN)

  • 0264-6021

PubMed ID

  • 1445255
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Additional Document Info

start page

  • 995

end page

  • 999

volume

  • 287

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