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Ligand profiling and identification technology for searching bioactive ligands

Academic Article
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Overview

authors

  • Baek, M. C.
  • Kim, S. J.
  • Yea, Kyungmoo
  • Kim, Y.
  • Lee, B. D.
  • Kim, J.
  • Lee, H. J.
  • Kang, M. H.
  • Choi, S. K.
  • Kim, J. I.
  • Lee, T. G.
  • Suh, P. G.
  • Ryu, S. H.

publication date

  • March 2006

journal

  • Proteomics  Journal

abstract

  • We introduce a new methodology named ligand profiling and identification for effective discovery of bioactive ligands such as peptide hormones. This technology was developed from a new concept of parallel column chromatography and active fraction profiling by nano-LC MS. Traditional methods use sequential column chromatography, and thus are inevitably limited by the low abundance of the peptide of interest and by a low yield due to the many column steps. Using this new technology, insulin was successfully identified and diarginylinsulin, a minor intermediate form of insulin, was unexpectedly also identified simultaneously from 100 mg of porcine pancreatic tissue. This integrative technology could be used to search for various low-abundance peptides (or bioactive molecules) rapidly and simultaneously, by applying this to the later stages of traditional sequential purification.

subject areas

  • Animals
  • Blotting, Western
  • Cells, Cultured
  • Chromatography, Gel
  • Chromatography, Ion Exchange
  • Chromatography, Liquid
  • Densitometry
  • Fibroblasts
  • Fluorescent Antibody Technique, Indirect
  • Humans
  • Insulin
  • Ligands
  • Mass Spectrometry
  • Nanotechnology
  • Pancreas
  • Peptide Hydrolases
  • Peptides
  • Rats
  • Receptor, Insulin
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Swine
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Research

keywords

  • identification
  • ligand
  • peptidome
  • profiling
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Identity

International Standard Serial Number (ISSN)

  • 1615-9853

Digital Object Identifier (DOI)

  • 10.1002/pmic.200500511

PubMed ID

  • 16526000
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Additional Document Info

start page

  • 1741

end page

  • 1749

volume

  • 6

issue

  • 6

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