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TALEN-mediated gene disruption on Y chromosome reveals critical role of EIF2S3Y in mouse spermatogenesis

Academic Article
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Overview

authors

  • Matsubara, Y.
  • Kato, T.
  • Kashimada, K.
  • Tanaka, H.
  • Zhi, Z.
  • Ichinose, S.
  • Mizutani, S.
  • Morio, T.
  • Chiba, T.
  • Ito, Y.
  • Saga, Y.
  • Takada, S.
  • Asahara, Hiroshi

publication date

  • May 2015

journal

  • Stem Cells and Development  Journal

abstract

  • The Y chromosome plays a critical role in spermatogenesis. Formerly, it had been difficult to generate knockout mice with specific Y chromosome mutations using conventional gene-targeting strategies. Recently, a transcription activator-like effector nuclease (TALEN) was successfully used for editing a mouse Y chromosome-linked gene. Here, we report the generation of a mouse model with a mutation in EIF2S3Y, a Y chromosome-linked gene, and analysis of its phenotype. The mouse carrying a targeted mutation of EIF2S3Y was infertile and had hypoplastic testes. Histological and electron microscopic analyses showed that differentiation of spermatogonia was arrested at the stage of spermatogonial stem cells (undifferentiated spermatogonia) and that the progression of spermatogenesis was interrupted, resulting in azoospermia. Using TALEN, we verified that EIF2S3Y performs a key function in differentiation of spermatogonial stem cells.

subject areas

  • Animals
  • Endonucleases
  • Eukaryotic Initiation Factor-2
  • Gene Targeting
  • Male
  • Mice
  • Mice, Knockout
  • Spermatogenesis
  • Spermatogonia
  • Testis
  • Y Chromosome
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Identity

PubMed Central ID

  • PMC4425228

International Standard Serial Number (ISSN)

  • 1547-3287

Digital Object Identifier (DOI)

  • 10.1089/scd.2014.0466

PubMed ID

  • 25579647
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Additional Document Info

start page

  • 1164

end page

  • 1170

volume

  • 24

issue

  • 10

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