Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Lambda foo: a lambda phage vector for the expression of foreign proteins

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Maruyama, I. N.
  • Maruyama, H. I.
  • Brenner, Sydney

publication date

  • August 1994

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • This work describes a lambda phage expression system, lambda foo, that produces foreign proteins fused to the surface of the virus particle. The lambda foo vector has multiple cloning sites for the insertion of a foreign DNA fragment and color selection for recombinants. Foreign proteins are fused to the C terminus of a truncated phage tail protein, pV, by a peptide linker. Conditional chain termination allows the assembly and fusion of multisubunit proteins. We have attached the complete Escherichia coli beta-galactosidase and the plant Bauhinia purpurea agglutinin by cloning their genes into the vector. The constructs express functionally active proteins on the phage particle surface and have been purified by affinity chromatography with an antibody for beta-galactosidase and a mucin as a ligand for Bauhinia purpurea agglutinin.

subject areas

  • Amino Acid Sequence
  • Bacteriophage lambda
  • Base Sequence
  • Carbohydrate Metabolism
  • Cloning, Molecular
  • DNA Primers
  • Escherichia coli
  • Gene Expression
  • Genetic Vectors
  • Immunoblotting
  • Lectins
  • Microscopy, Electron
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Plant Lectins
  • Polymerase Chain Reaction
  • Recombinant Fusion Proteins
  • Recombinant Proteins
  • Restriction Mapping
  • Substrate Specificity
  • Templates, Genetic
  • beta-Galactosidase
scroll to property group menus

Research

keywords

  • BAUHINIA PURPUREA AGGLUTININ
  • BETA-GALACTOSIDASE
  • CONDITIONAL FUSION
  • SITE-DIRECTED MUTAGENESIS
  • V GENE
scroll to property group menus

Identity

PubMed Central ID

  • PMC44588

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.91.17.8273

PubMed ID

  • 8058794
scroll to property group menus

Additional Document Info

start page

  • 8273

end page

  • 8277

volume

  • 91

issue

  • 17

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support