Cell-free expression of functional receptor tyrosine kinases

Academic Article

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Overview

authors

• He, W.
• Scharadin, T. M.
• Saldana, M.
• Gellner, C.
• Hoang-Phou, S.
• Takanishi, C.
• Hura, G. L.
• Tainer, John
• Carraway III, K. L.
• Henderson, P. T.
• Coleman, M. A.

publication date

• August 2015

journal

• Scientific Reports  Journal

abstract

• Receptor tyrosine kinases (RTKs) play critical roles in physiological and pathological processes, and are important anticancer drug targets. In vitro mechanistic and drug discovery studies of full-length RTKs require protein that is both fully functional and free from contaminating proteins. Here we describe a rapid cell-free and detergent-free co-translation method for producing full-length and functional ERBB2 and EGFR receptor tyrosine kinases supported by water-soluble apolipoprotein A-I based nanolipoprotein particles.

subject areas

• Cell-Free System
• Receptor Protein-Tyrosine Kinases

Identity

PubMed Central ID

• PMC4929682

International Standard Serial Number (ISSN)

• 2045-2322

Digital Object Identifier (DOI)

• 10.1038/srep12896

PubMed ID

• 26274523

Additional Document Info

start page

• 012896

volume

• 5