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Cell-free expression of functional receptor tyrosine kinases

Academic Article
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Overview

authors

  • He, W.
  • Scharadin, T. M.
  • Saldana, M.
  • Gellner, C.
  • Hoang-Phou, S.
  • Takanishi, C.
  • Hura, G. L.
  • Tainer, John
  • Carraway III, K. L.
  • Henderson, P. T.
  • Coleman, M. A.

publication date

  • August 2015

journal

  • Scientific Reports  Journal

abstract

  • Receptor tyrosine kinases (RTKs) play critical roles in physiological and pathological processes, and are important anticancer drug targets. In vitro mechanistic and drug discovery studies of full-length RTKs require protein that is both fully functional and free from contaminating proteins. Here we describe a rapid cell-free and detergent-free co-translation method for producing full-length and functional ERBB2 and EGFR receptor tyrosine kinases supported by water-soluble apolipoprotein A-I based nanolipoprotein particles.

subject areas

  • Cell-Free System
  • Receptor Protein-Tyrosine Kinases
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Identity

PubMed Central ID

  • PMC4929682

International Standard Serial Number (ISSN)

  • 2045-2322

Digital Object Identifier (DOI)

  • 10.1038/srep12896

PubMed ID

  • 26274523
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Additional Document Info

start page

  • 012896

volume

  • 5

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