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Generation of cell lines to study the role played by oncogenes and anti-oncogenes in cell proliferation control

Academic Article
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Overview

authors

  • Costanzi, E.
  • Rameh, L. E.
  • Joazeiro, Claudio Antonio
  • Armelin, M. C. S.

publication date

  • 1990

journal

  • Brazilian Journal of Medical and Biological Research  Journal

abstract

  • We report the generation of stable transfectant cell lines by DNA-mediated transfection that overexpress viral and/or cellular oncogenes. Expression of heterologous genes (FBJ- and FBR-v-fos, polyoma large and middle T) was confirmed by Northern hybridization, immunofluorescence and immunoprecipitation. We also describe the isolation of two retinoblastoma cell lines from human tumors. Neuronal and glial markers were used to confirm the origin of these cell lines. Oncogene transfectant and retinoblastoma cell lines will be used to assess Rb expression and the possible role of its gene product in cell proliferation control and neoplasia.

subject areas

  • Cell Division
  • Cell Line, Transformed
  • Gene Expression Regulation, Neoplastic
  • Genes, Tumor Suppressor
  • Humans
  • Oncogenes
  • Phenotype
  • Retinoblastoma
  • Transfection
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Identity

International Standard Serial Number (ISSN)

  • 0100-879X

PubMed ID

  • 2101319
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Additional Document Info

start page

  • 795

end page

  • 799

volume

  • 23

issue

  • 9

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