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Worker honeybee brain proteome

Academic Article
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Overview

authors

  • Hernandez, L. G.
  • Lu, B.
  • da Cruz, G. C. N.
  • Calabria, L. K.
  • Martins, N. F.
  • Togawa, R.
  • Espindola, F. S.
  • Yates III, John
  • Cunha, R. B.
  • de Sousa, M. V.

publication date

  • March 2012

journal

  • Journal of Proteome Research  Journal

abstract

  • A large-scale mapping of the worker honeybee brain proteome was achieved by MudPIT. We identified 2742 proteins from forager and nurse honeybee brain samples; 17% of the total proteins were found to be differentially expressed by spectral count sampling statistics and a G-test. Sequences were compared with the EuKaryotic Orthologous Groups (KOG) catalog set using BLASTX and then categorized into the major KOG categories of most similar sequences. According to this categorization, nurse brain showed increased expression of proteins implicated in translation, ribosomal structure, and biogenesis (14.5%) compared with forager (1.8%). Experienced foragers overexpressed proteins involved in energy production and conversion, showing an extensive difference in this set of proteins (17%) in relation to the nurse subcaste (0.6%). Examples of proteins selectively expressed in each subcaste were analyzed. A comparison between these MudPIT experiments and previous 2-DE experiments revealed nine coincident proteins differentially expressed in both methodologies.

subject areas

  • Animals
  • Bees
  • Brain
  • Chromatography, High Pressure Liquid
  • Electrophoresis, Gel, Two-Dimensional
  • Gene Expression
  • Gene Expression Profiling
  • Insect Proteins
  • Protein Isoforms
  • Proteome
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Research

keywords

  • Apis mellifera
  • MudPIT
  • brain proteome
  • differential expression
  • honeybee subcastes
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Identity

PubMed Central ID

  • PMC3321609

International Standard Serial Number (ISSN)

  • 1535-3893

Digital Object Identifier (DOI)

  • 10.1021/pr2007818

PubMed ID

  • 22181811
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Additional Document Info

start page

  • 1485

end page

  • 1493

volume

  • 11

issue

  • 3

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