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Repair of cartilage defects in arthritic tissue with differentiated human embryonic stem cells

Academic Article
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Overview

authors

  • Olee, T.
  • Grogan, S. P.
  • Lotz, Martin
  • Colwell Jr., Clifford
  • D'Lima, Darryl
  • Snyder, E. Y.

publication date

  • February 2014

journal

  • Tissue Engineering Part A  Journal

abstract

  • Chondrocytes have been generated in vitro from a range of progenitor cell types and by a number of strategies. However, achieving reconstitution of actual physiologically relevant, appropriately-laminated cartilage in situ that would be applicable to conditions, such as arthritis and cartilage degeneration remains elusive. This lack of success is multifactorial and includes limited cell source, decreased proliferation rate of mature chondrocytes, lack of maintenance of phenotype, reduced matrix synthesis, and poor integration with host tissue. We report an efficient approach for deriving mesenchymal chondroprogenitor cells from human embryonic stem cells. These cells generated tissue containing cartilage-specific matrix proteins that integrated in situ in a partial-thickness defect in ex vivo articular cartilage harvested from human arthritic joints. Given that stem cells provide a virtually inexhaustible supply of starting material and that our technique is easily scalable, cartilaginous tissue primed and grafted in this manner could be suitable for clinical translation.

subject areas

  • Arthritis
  • Biomarkers
  • Cartilage, Articular
  • Cell Differentiation
  • Cell Line
  • Chondrogenesis
  • Collagen Type II
  • Embryonic Stem Cells
  • Fluorescent Antibody Technique
  • Gene Expression Regulation
  • Glycoproteins
  • Humans
  • Mesenchymal Stem Cells
  • Pluripotent Stem Cells
  • Stem Cell Transplantation
  • Wound Healing
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Identity

PubMed Central ID

  • PMC3926163

International Standard Serial Number (ISSN)

  • 1937-3341

Digital Object Identifier (DOI)

  • 10.1089/ten.tea.2012.0751

PubMed ID

  • 24028447
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Additional Document Info

start page

  • 683

end page

  • 692

volume

  • 20

issue

  • 3-4

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