recent publications
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academic article
- Unwin, N., Fujiyoshi, Y. Gating movement of acetylcholine receptor caught by plunge-freezing Journal of Molecular Biology 2012 422:617-634 DOI:10.1016/j.jmb.2012.07.010 PMID:22841691 PMCID:PMC3443390
- Fisher, L. S., Ward, A., Milligan, R. A., Unwin, N., Potter, C. S., Carragher, B. A helical processing pipeline for EM structure determination of membrane proteins Methods 2011 55:350-362 DOI:10.1016/j.ymeth.2011.09.013 PMID:21964395 PMCID:PMC3262078
- Fujiyoshi, Y., Unwin, N. Electron crystallography of proteins in membranes Current Opinion in Structural Biology 2008 18:587-592 DOI:10.1016/j.sbi.2008.07.005 PMID:18755273 PMCID:PMC2651081
- O'Brien, J., Unwin, N. Organization of spines on the dendrites of purkinje cells Proceedings of the National Academy of Sciences of the United States of America 2006 103:1575-1580 DOI:10.1073/pnas.0507884103 PMID:16423897 PMCID:PMC1360541
- Unwin, N. Refined structure of the nicotinic acetylcholine receptor at 4 Å resolution Journal of Molecular Biology 2005 346:967-989 DOI:10.1016/j.jmb.2004.12.031 PMID:15701510
- Unwin, N. Structure and action of the nicotinic acetylcholine receptor explored by electron microscopy FEBS Letters 2003 555:91-95 DOI:10.1016/s0014-5793(03)01084-6 PMID:14630325
- Miyazawa, A., Fujiyoshi, Y., Unwin, N. Structure and gating mechanism of the acetylcholine receptor pore Nature 2003 423:949-955 DOI:10.1038/nature01748 PMID:12827192
- Unwin, N., Miyazawa, A., Li, J., Fujiyoshi, Y. Activation of the nicotinic acetylcholine receptor involves a switch in conformation of the a subunits Journal of Molecular Biology 2002 319:1165-1176 DOI:10.1016/s0022-2836(02)00381-9 PMID:12079355
- Unwin, N. The croonian lecture 2000. Nicotinic acetylcholine receptor and the structural basis of fast synaptic transmission Philosophical Transactions of the Royal Society of London Series B-Biological Sciences 2000 355:1813-1829 DOI:10.1098/rstb.2000.0737 PMID:11205343 PMCID:PMC1692909
- Tierney, M. L., Unwin, N. Electron microscopic evidence for the assembly of soluble pentameric extracellular domains of the nicotinic acetylcholine receptor Journal of Molecular Biology 2000 303:185-196 DOI:10.1006/jmbi.2000.4137 PMID:11023785
- Miyazawa, A., Fujiyoshi, Y., Stowell, M., Unwin, N. Nicotinic acetylcholine receptor at 4.6 Å resolution: Transverse tunnels in the channel wall Journal of Molecular Biology 1999 288:765-786 DOI:10.1006/jmbi.1999.2721 PMID:10329178
- Stowell, M. H. B., Miyazawa, A., Unwin, N. Macromolecular structure determination by electron microscopy: New advances and recent results Current Opinion in Structural Biology 1998 8:595-600 DOI:10.1016/s0959-440x(98)80150-4 PMID:9818263
- Unwin, N. The nicotinic acetylcholine receptor of the torpedo electric ray Journal of Structural Biology 1998 121:181-190 DOI:10.1006/jsbi.1997.3949 PMID:9615437
- Beroukhim, R., Unwin, N. Distortion correction of tubular crystals: Improvements in the acetylcholine receptor structure Ultramicroscopy 1997 70:57-81 DOI:10.1016/s0304-3991(97)00070-3 PMID:9440347
- Unwin, N. Projection structure of the nicotinic acetylcholine receptor: Distinct conformations of the alpha subunits Journal of Molecular Biology 1996 257:586-596 DOI:10.1006/jmbi.1996.0187 PMID:8648626
- Beroukhim, R., Unwin, N. Three-dimensional location of the main immunogenic region of the acetylcholine receptor Neuron 1995 15:323-331 DOI:10.1016/0896-6273(95)90037-3 PMID:7544140
- Unwin, N. Acetylcholine receptor channel imaged in the open state Nature 1995 373:37-43 DOI:10.1038/373037a0 PMID:7800037
- Berriman, J., Unwin, N. Analysis of transient structures by cryomicroscopy combined with rapid mixing of spray droplets Ultramicroscopy 1994 56:241-252 DOI:10.1016/0304-3991(94)90012-4 PMID:7831735
- Li, M., Unwin, N., Stauffer, K. A., Jan, Y. N., Jan, L. Y. Images of purified shaker potassium channels Current Biology 1994 4:110-115 DOI:10.1016/s0960-9822(94)00026-6 PMID:7953509
- Unwin, N. Nicotinic acetylcholine-receptor at 9 Å resolution Journal of Molecular Biology 1993 229:1101-1124 DOI:10.1006/jmbi.1993.1107 PMID:8445638
- Unwin, N. Neurotransmitter action - opening of ligand-gated ion channels Cell 1993 72:31-41 DOI:10.1016/s0092-8674(05)80026-1 PMID:7679054
- Hyman, A. A., Salser, S., Drechsel, D. N., Unwin, N., Mitchison, T. J. Role of gtp hydrolysis in microtubule dynamics - information from a slowly hydrolyzable analog, gmpcpp Molecular Biology of the Cell 1992 3:1155-1167 PMID:1421572 PMCID:PMC275679
- Stauffer, K. A., Kumar, N. M., Gilula, N. B., Unwin, N. Isolation and purification of gap junction channels Journal of Cell Biology 1991 115:141-150 DOI:10.1083/jcb.115.1.141 PMID:1655801
- Toyoshima, C., Unwin, N. Three-dimensional structure of the acetylcholine receptor by cryoelectron microscopy and helical image reconstruction Journal of Cell Biology 1990 111:2623-2635 DOI:10.1083/jcb.111.6.2623 PMID:2277076 PMCID:PMC2116367
- Unwin, N. The structure of ion channels in membranes of excitable cells Neuron 1989 3:665-676 DOI:10.1016/0896-6273(89)90235-3 PMID:2484344
- Toyoshima, C., Unwin, N. Ion channel of acetylcholine-receptor reconstructed from images of postsynaptic membranes Nature 1988 336:247-250 DOI:10.1038/336247a0 PMID:2461515
- Milks, L. C., Kumar, N. M., Houghten, R., Unwin, N., Gilula, N. B. Topology of the 32-kd liver gap junction protein determined by site-directed antibody localizations EMBO Journal 1988 7:2967-2975 PMID:2460334 PMCID:PMC454678
- Unwin, N., Toyoshima, C., Kubalek, E. Arrangement of the acetylcholine-receptor subunits in the resting and desensitized states, determined by cryoelectron microscopy of crystallized torpedo postsynaptic membranes Journal of Cell Biology 1988 107:1123-1138 DOI:10.1083/jcb.107.3.1123 PMID:3417777
- Gogol, E., Unwin, N. Organization of connexons in isolated rat-liver gap-junctions Biophysical Journal 1988 54:105-112 DOI:10.1016/S0006-3495(88)82935-7 PMID:3416022 PMCID:PMC1330320
- Sikerwar, S. S., Unwin, N. Three-dimensional structure of gap junctions in fragmented plasma membranes from rat liver Biophysical Journal 1988 54:113-119 DOI:10.1016/S0006-3495(88)82936-9 PMID:3416023 PMCID:PMC1330321
- Lyon, M. K., Unwin, P. N. T. Two-dimensional structure of the light-harvesting chlorophyll a/b complex by cryoelectron microscopy Journal of Cell Biology 1988 106:1515-1523 DOI:10.1083/jcb.106.5.1515 PMID:2453515 PMCID:PMC2115055
- Toyoshima, C., Unwin, N. Contrast transfer for frozen-hydrated specimens - determination from pairs of defocused images Ultramicroscopy 1988 25:279-291 DOI:10.1016/0304-3991(88)90003-4 PMID:3188279
- Unwin, N. Design and action of cell communication channels Chemica Scripta 1987 27B:47-51
- Kubalek, E., Ralston, S., Lindstrom, J., Unwin, N. Location of subunits within the acetylcholine-receptor by electron image-analysis of tubular crystals from torpedo-marmorata Journal of Cell Biology 1987 105:9-18 DOI:10.1083/jcb.105.1.9 PMID:3611197
- Unwin, P. N. T. Gap junction structure and the control of cell-to-cell communication Ciba Foundation Symposia 1987 125:78-91 PMID:2435472
- Unwin, N. The use of cryoelectron microscopy in elucidating molecular design and mechanisms Annals of the New York Academy of Sciences 1986 483:1-4 DOI:10.1111/j.1749-6632.1986.tb34485.x PMID:3471117
- Milligan, R. A., Unwin, P. N. T. Location of exit channel for nascent protein in 80S ribosome Nature 1986 319:693-695 DOI:10.1038/319693a0 PMID:3951541
- Brisson, A., Unwin, P. N. T. Quaternary structure of the acetylcholine-receptor Nature 1985 315:474-477 DOI:10.1038/315474a0 PMID:4000275
- Taylor, K. A., Milligan, R. A., Raeburn, C., Unwin, P. N. T. A cold stage for the philips em300 electron-microscope Ultramicroscopy 1984 13:185-189 DOI:10.1016/0304-3991(84)90197-9 PMID:6485128
- Milligan, R. A., Brisson, A., Unwin, P. N. T. Molecular-structure determination of crystalline specimens in frozen aqueous-solutions Ultramicroscopy 1984 13:1-9 DOI:10.1016/0304-3991(84)90051-2 PMID:6474593
- Unwin, N., Henderson, R. The structure of proteins in biological membranes Scientific American 1984 250:78-94 PMID:6318309
- Brisson, A., Unwin, P. N. T. Tubular crystals of acetylcholine-receptor Journal of Cell Biology 1984 99:1202-1211 DOI:10.1083/jcb.99.4.1202 PMID:6480689
- Unwin, P. N. T., Ennis, P. D. Two configurations of a channel-forming membrane protein Nature 1984 307:609-613 DOI:10.1038/307609a0 PMID:6320017
- Unwin, P. N. T., Ennis, P. D. Calcium-mediated changes in gap junction structure - evidence from the low-angle x-ray pattern Journal of Cell Biology 1983 97:1459-1466 DOI:10.1083/jcb.97.5.1459 PMID:6630291
- Amos, L. A., Henderson, R., Unwin, P. N. T. Three-dimensional structure determination by electron microscopy of two-dimensional crystals Progress in Biophysics & Molecular Biology 1983 39:183-231 DOI:10.1016/0079-6107(83)90017-2 PMID:6289376
- Unwin, P. N. T., Milligan, R. A. A large particle associated with the perimeter of the nuclear-pore complex Journal of Cell Biology 1982 93:63-75 DOI:10.1083/jcb.93.1.63 PMID:7068761
- Kuhlbrandt, W., Unwin, P. N. T. Distribution of rna and protein in crystalline eukaryotic ribosomes Journal of Molecular Biology 1982 156:431-448 DOI:10.1016/0022-2836(82)90259-5 PMID:6811751
- Milligan, R. A., Unwin, P. N. T. In vitro crystallization of ribosomes from chick embryos Journal of Cell Biology 1982 95:648-653 DOI:10.1083/jcb.95.2.648 PMID:7142299 PMCID:PMC2112976
- Unwin, P. N. T., Zampighi, G. Structure of the junction between communicating cells Nature 1980 283:545-549 DOI:10.1038/283545a0 PMID:7354837
- Unwin, P. N. T. Attachment of ribosome crystals to intracellular membranes Journal of Molecular Biology 1979 132:69-84 DOI:10.1016/0022-2836(79)90496-0 PMID:117113
- Zampighi, G., Unwin, P. N. T. Two forms of isolated gap junctions Journal of Molecular Biology 1979 135:451-464 DOI:10.1016/0022-2836(79)90446-7 PMID:537084
- Unwin, P. N. T., Taddei, C. Packing of ribosomes in crystals from lizard lacerta-sicula Journal of Molecular Biology 1977 114:491-506 DOI:10.1016/0022-2836(77)90174-7 PMID:562417
- Henderson, R., Unwin, P. N. T. Structure of purple membrane from halobacterium-halobium Biophysics of Structure and Mechanism 1977 3:121-121 DOI:10.1007/bf00535804 PMID:890047
- Unwin, P. N. T. Three-dimensional model of membrane-bound ribosomes obtained by electron microscopy Nature 1977 269:118-122 DOI:10.1038/269118a0 PMID:909577
- Mandelkow, E. M., Mandelkow, E., Unwin, N., Cohen, C. Tubulin hoops Nature 1977 265:655-657 DOI:10.1038/265655a0 PMID:859570
- Unwin, P. N. T. Beef liver catalase structure - interpretation of electron micrographs Journal of Molecular Biology 1975 98:235-242 DOI:10.1016/s0022-2836(75)80111-2 PMID:1195381
- Unwin, P. N. T., Henderson, R. Molecular structure determination by electron microscopy of unstained crystalline specimens Journal of Molecular Biology 1975 94:425-440 DOI:10.1016/0022-2836(75)90212-0 PMID:1236957
- Henderson, R., Unwin, P. N. T. Three-dimensional model of purple membrane obtained by electron microscopy Nature 1975 257:28-32 DOI:10.1038/257028a0 PMID:1161000
- Unwin, P. N. T. Electron microscopy of the stacked disk aggregate of tobacco mosaic virus protein. Ii. The influence of electron irradiation of the stain distribution Journal of Molecular Biology 1974 87:657-670 DOI:10.1016/0022-2836(74)90076-x PMID:4139268
- Unwin, P. N. T., Klug, A. Electron microscopy of the stacked disk aggregate of tobacco mosaic-virus protein: 1. Three-dimensional image reconstruction Journal of Molecular Biology 1974 87:641-650 DOI:10.1016/0022-2836(74)90075-8 PMID:4427373
- Unwin, P. N. T. New electron-microscope imaging method for enhancing detail in thin biological specimens Zeitschrift für Naturforschung Section A-A Journal of Physical Sciences 1974 A 29:158-163
- Unwin, P. N. T. Phase-contrast electron-microscopy of biological-materials Journal of Microscopy 1973 98:299-312 PMID:4126993
- Unwin, P. N. T. Electron microscopy of biological specimens by means of an electrostatic phase plate Proceedings of the Royal Society of London Series a-Mathematical and Physical Sciences 1972 329:327-359 DOI:10.1098/rspa.1972.0116
- Unwin, P. N. T., Muguruma, J. Electron-microscope observations on defect structure of ice Physica Status Solidi a-Applied Research 1972 14:207-216 DOI:10.1002/pssa.2210140125
- Unwin, P. N. T. Phase contrast and interference microscopy with the electron microscope Philosophical Transactions of the Royal Society of London Series B-Biological Sciences 1971 261:95-104 DOI:10.1098/rstb.1971.0039 PMID:4399209
- Unwin, P. N. T., Muguruma, J. Transmission electron microscopy of ice Journal of Applied Physics 1971 42:3640-3641 DOI:10.1063/1.1660782
- Unwin, P. N. T. An electrostatic phase plate for the electron microscope Berichte Der Bunsen-Gesellschaft Fur Physikalische Chemie 1970 74:1137-1141 DOI:10.1002/bbpc.19700741110
- Unwin, P. N. T., Wilkins, M. A. A technique for the preparation of thin foils from regions near cracks in massive bodies Journal of Physics E-Scientific Instruments 1969 2:736 DOI:10.1088/0022-3735/2/8/435
- Unwin, P. N. T., Smith, G. C. Microstructure and mechanical properties of al-6% zn-3% mg Journal of the Institute of Metals 1969 97:299-310
- Unwin, P. N. T., Nicholson, R. B. The nucleation and initial stages of growth of grain boundary precipitates in al-zn-mg and al-mg alloys Acta Metallurgica 1969 17:1379-1393 DOI:10.1016/0001-6160(69)90155-2
- Unwin, P. N. T., Lorimer, G. W., Nicholson, R. B. The origin of the grain boundary precipitate free zone Acta Metallurgica 1969 17:1363-1377 DOI:10.1016/0001-6160(69)90154-0
- Cundy, S. L., Metherell, A. J. F., Nicholson, R. B., Unwin, P. N. T., Whelan, M. J. Studies of segregation and the initial stages of precipitation at grain boundaries in an aluminium 7 wt. Percent magnesium alloy with an energy analysing electron microscope Proceedings of the Royal Society of London Series a-Mathematical and Physical Sciences 1968 307:267-281 DOI:10.1098/rspa.1968.0189