recent publications
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academic article
- Ilinykh, P. A., Huang, K., Santos, R. I., Gilchuk, P., Gunn, B. M., Karim, M. M., Liang, J., Fouch, M. E., Davidson, E., Parekh, D. V., Kimble, J. B., Pietzsch, C. A., et al. Non-neutralizing Antibodies from a Marburg Infection Survivor Mediate Protection by Fc-Effector Functions and by Enhancing Efficacy of Other Antibodies Cell Host & Microbe 2020 27:976-991 DOI:10.1016/j.chom.2020.03.025 PMID:32320678 PMCID:PMC7292764
- Gilchuk, P., Murin, C. D., Milligan, J. C., Cross, R. W., Mire, C. E., Ilinykh, P. A., Huang, K., Kuzmina, N., Altman, P. X., Hui, S., Gunn, B. M., Bryan, A. L., et al. Analysis of a therapeutic antibody cocktail reveals determinants for cooperative and broad ebolavirus neutralization Immunity 2020 52:388-403 DOI:10.1016/j.immuni.2020.01.001 PMID:32023489
- Lin, A. E., Diehl, W. E., Cai, Y., Finch, C. L., Akusobi, C., Kirchdoerfer, R. N., Bollinger, L., Schaffner, S. F., Brown, E. A., Saphire, E. O., Andersen, K. G., Kuhn, J. H., et al. Reporter assays for Ebola virus nucleoprotein oligomerization, virion-like particle budding, and minigenome activity reveal the importance of nucleoprotein amino acid position 111 Viruses-Basel 2020 12 DOI:10.3390/v12010105 PMID:31952352 PMCID:PMC7019320
- Hastie, K. M., Cross, R. W., Harkins, S. S., Zandonatti, M. A., Koval, A. P., Heinrich, M. L., Rowland, M. M., Robinson, J. E., Geisbert, T. W., Garry, R. F., Branco, L. M., Saphire, E. O. Convergent structures illuminate features for germline antibody binding and pan-Lassa virus neutralization Cell 2019 178:1004-1015 DOI:10.1016/j.cell.2019.07.020 PMID:31398326
- Landeras-Bueno, S., Oda, S. I., Norris, M. J., Li Salie, Z., Guenaga, J., Wyatt, R. T., Saphire, E. O. Sudan Ebolavirus VP35-NP crystal structure reveals a potential target for pan-filovirus treatment MBio 2019 10:e00734-19 DOI:10.1128/mBio.00734-19 PMID:31337716 PMCID:PMC6650547
- Davis, C. W., Jackson, K. J. L., McElroy, A. K., Halfmann, P., Huang, J., Chennareddy, C., Piper, A. E., Leung, Y., Albarino, C. G., Crozier, I., Ellebedy, A. H., Sidney, J., et al. Longitudinal analysis of the human B cell response to Ebola virus infection Cell 2019 177:1566-1582 DOI:10.1016/j.cell.2019.04.036 PMID:31104840
- Kirchdoerfer, R. N., Saphire, E. O., Ward, A. B. Cryo-EM structure of the Ebola virus nucleoprotein-RNA complex Acta Crystallographica Section F-Structural Biology Communications 2019 75:340-347 DOI:10.1107/s2053230x19004424 PMID:31045563 PMCID:PMC6497097
- King, L. B.*, West, B. R.*, Moyer, C. L., Gilchuk, P., Flyak, A., Ilinykh, P. A., Bombardi, R., Hui, S., Huang, K., Bukreyev, A., Crowe, J. E., Saphire, E. O. Cross-reactive neutralizing human survivor monoclonal antibody BDBV223 targets the ebolavirus stalk Nature Communications 2019 10 DOI:10.1038/s41467-019-09732-7 PMID:30996276 PMCID:PMC6470140 (* = equal contribution)
- Bruhn, J. F., Hotard, A. L., Spiropoulou, C. F., Lo, M. K., Saphire, E. O. A conserved basic patch and central kink in the nipah virus phosphoprotein multimerization domain are essential for polymerase function Structure 2019 27:660-668 DOI:10.1016/j.str.2019.01.012 PMID:30799076
- Williamson, L. E., Flyak, A. I., Kose, N., Bombardi, R., Branchizio, A., Reddy, S., Davidson, E., Doranz, B. J., Fusco, M. L., Saphire, E. O., Hoffmann, P. J., Kawaoka, Y., et al. Early human B cell response to Ebola virus in four US survivors of infection Journal of Virology 2019 93 DOI:10.1128/jvi.01439-18 PMID:30728263 PMCID:PMC6450119
- West, B. R., Wec, A. Z., Moyer, C. L., Fusco, M. L., Ilinykh, P. A., Huang, K., Wirchnianski, A. S., James, R. M., Herbert, A. S., Hui, S., Goodwin, E., Howell, K. A., et al. Structural basis of broad ebolavirus neutralization by a human survivor antibody Nature Structural & Molecular Biology 2019 26:204-212 DOI:10.1038/s41594-019-0191-4 PMID:30833785 PMCID:PMC6402988
- King, L. B., Milligan, J. C., West, B. R., Schendel, S. L., Saphire, E. O. Achieving cross-reactivity with pan-ebolavirus antibodies Current Opinion in Virology 2019 34:140-148 DOI:10.1016/j.coviro.2019.01.003 PMID:30884329
- Milligan, J. C., Parekh, D. V., Fuller, K. M., Igarashi, M., Takada, A., Saphire, E. O. Structural characterization of pan-Ebolavirus antibody 6D6 targeting the fusion peptide of the surface glycoprotein Journal of Infectious Diseases 2019 219:415-419 DOI:10.1093/infdis/jiy532 PMID:30203042 PMCID:PMC6325343
- Bramble, M. S., Hoff, N., Gilchuk, P., Mukadi, P., Lu, K., Doshi, R. H., Steffen, I., Nicholson, B. P., Lipson, A., Vashist, N., Sinai, C., Spencer, D., et al. Pan-filovirus serum neutralizing antibodies in a subset of Congolese ebolavirus infection survivors Journal of Infectious Diseases 2018 218:1929-1936 DOI:10.1093/infdis/jiy453 PMID:30107445 PMCID:PMC6217721
- Patel, A., Park, D. H., Davis, C. W., Smith, T. R. F., Leung, A., Tierney, K., Bryan, A., Davidson, E., Yu, X., Racine, T., Reed, C., Gorman, M. E., et al. In vivo delivery of synthetic human DNA-encoded monoclonal antibodies protect against ebolavirus infection in a mouse model Cell Reports 2018 25:1982-1993 DOI:10.1016/j.celrep.2018.10.062 PMID:30428362
- Saphire, E. O., Schendel, S. L., Gunn, B. M., Milligan, J. C., Alter, G. Antibody-mediated protection against Ebola virus Nature Immunology 2018 19:1169-1178 DOI:10.1038/s41590-018-0233-9 PMID:30333617
- West, B. R., Moyer, C. L., King, L. B., Fusco, M. L., Milligan, J. C., Hui, S., Saphire, E. O. Structural basis of pan-ebolavirus neutralization by a human antibody against a conserved, yet cryptic epitope MBio 2018 9:e01674-18 DOI:10.1128/mBio.01674-18 PMID:30206174 PMCID:PMC6134094
- Gilchuk, P., Kuzmina, N., Ilinykh, P. A., Huang, K., Gunn, B. M., Bryan, A., Davidson, E., Doranz, B. J., Turner, H. L., Fusco, M. L., Bramble, M. S., Hoff, N. A., et al. Multifunctional pan-ebolavirus antibody recognizes a site of broad vulnerability on the ebolavirus glycoprotein Immunity 2018 49:363-374 DOI:10.1016/j.immuni.2018.06.018 PMID:30029854 PMCID:PMC6104738
- Meyer, M., Yoshida, A., Ramanathan, P., Saphire, E. O., Collins, P. L., Crowe, J. E., Samal, S., Bukreyev, A. Antibody repertoires to the same Ebola vaccine antigen are differentially affected by vaccine vectors Cell Reports 2018 24:1816-1829 DOI:10.1016/j.celrep.2018.07.044 PMID:30110638
- Saphire, E. O., Schendel, S. L., Fusco, M. L., Gangavarapu, K., Gunn, B. M., Wec, A. Z., Halfmann, P. J., Brannan, J. M., Herbert, A. S., Qiu, X. G., Wagh, K., He, S., et al. Systematic analysis of monoclonal antibodies against Ebola virus GP defines features that contribute to protection Cell 2018 174:938-952 DOI:10.1016/j.cell.2018.07.033 PMID:30096313 PMCID:PMC6102396
- Gunn, B. M., Yu, W. H., Karim, M. M., Brannan, J. M., Herbert, A. S., Wec, A. Z., Halfmann, P. J., Fusco, M. L., Schendel, S. L., Gangavarapu, K., Krause, T., Qiu, X., et al. A role for Fc function in therapeutic monoclonal antibody-mediated protection against Ebola virus Cell Host & Microbe 2018 24:221-233 DOI:10.1016/j.chom.2018.07.009 PMID:30092199
- Hastie, K. M., Saphire, E. O. Lassa virus glycoprotein: stopping a moving target Current Opinion in Virology 2018 31:52-58 DOI:10.1016/j.coviro.2018.05.002 PMID:29843991 PMCID:PMC6193841
- King, L. B.*, West, B. R.*, Schendel, S. L., Saphire, E. O. The structural basis for filovirus neutralization by monoclonal antibodies Curr Opin Immunol 2018 53:196-202 DOI:10.1016/j.coi.2018.05.001 PMID:29940415 (* = equal contribution)
- Flyak, A. I., Kuzmina, N., Murin, C. D., Bryan, C., Davidson, E., Gilchuk, P., Gulka, C. P., Ilinykh, P. A., Shen, X. L., Huang, K., Ramanathan, P., Turner, H., et al. Broadly neutralizing antibodies from human survivors target a conserved site in the Ebola virus glycoprotein HR2-MPER region Nature Microbiology 2018 3:670-677 DOI:10.1038/s41564-018-0157-z PMID:29736037
- Amanat, F., Duehr, J., Oestereich, L., Hastie, K. M., Saphire, E. O., Krammer, F. Antibodies to the glycoprotein GP2 subunit cross-react between old and new world arenaviruses mSphere 2018 3:e00189-18 DOI:10.1128/mSphere.00189-18 PMID:29720525 PMCID:PMC5932378
- Boisen, M. L., Hartnett, J. N., Shaffer, J. G., Goba, A., Momoh, M., Sandi, J. D., Fullah, M., Nelson, D. K. S., Bush, D. J., Rowland, M. M., Heinrich, M. L., Koval, A. P., et al. Field validation of recombinant antigen immunoassays for diagnosis of Lassa fever Scientific Reports 2018 8 DOI:10.1038/s41598-018-24246-w PMID:29651117 PMCID:PMC5897328
- Del Vecchio, K., Frick, C. T., Gc, J. B., Oda, S. I., Gerstman, B. S., Saphire, E. O., Chapagain, P. P., Stahelin, R. V. A cationic, C-terminal patch and structural rearrangements in Ebola virus matrix VP40 protein control its interactions with phosphatidylserine Journal of Biological Chemistry 2018 293:3335-3349 DOI:10.1074/jbc.M117.816280 PMID:29348171 PMCID:PMC5836117
- King, L. B., Fusco, M. L., Flyak, A. I., Ilinykh, P. A., Huang, K., Gunn, B., Kirchdoerfer, R. N., Hastie, K. M., Sangha, A. K., Meiler, J., Alter, G., Bukreyev, A., et al. The Marburgvirus-neutralizing human monoclonal antibody MR191 targets a conserved site to block virus receptor binding Cell Host & Microbe 2018 23:101-109 DOI:10.1016/j.chom.2017.12.003 PMID:29324225 PMCID:PMC5772738
- Sangha, A. K., Dong, J., Williamson, L., Hashiguchi, T., Saphire, E. O., Crowe, J. E., Meiler, J. Role of non-local interactions between CDR loops in binding affinity of MR78 antibody to Marburg virus glycoprotein Structure 2017 25:1820-1828 DOI:10.1016/j.str.2017.10.005 PMID:29153506 PMCID:PMC5718948
- Galan-Navarro, C., Rincon-Restrepo, M., Zimmer, G., Saphire, E. O., Hubbell, J. A., Hirosue, S., Swartz, M. A., Kunz, S. Oxidation-sensitive polymersomes as vaccine nanocarriers enhance humoral responses against Lassa virus envelope glycoprotein Virology 2017 512:161-171 DOI:10.1016/j.virol.2017.09.013 PMID:28963882
- Hastie, K. M., Zandonatti, M. A., Kleinfelter, L. M., Heinrich, M. L., Rowland, M. M., Chandran, K., Branco, L. M., Robinson, J. E., Garry, R. F., Saphire, E. O. Structural basis for antibody-mediated neutralization of Lassa virus Science 2017 356:923-928 DOI:10.1126/science.aam7260 PMID:28572385
- Saphire, E. O., Dye, J. M., Kobinger, G. P., Zeitlin, L., Chandran, K., Garry, R. F. How to turn competitors into collaborators Nature 2017 541:283-285 PMID:28102273
- Bruhn, J. F., Kirchdoerfer, R. N., Urata, S. M., Li, S., Tickle, I. J., Bricogne, G., Saphire, E. O. Crystal structure of the Marburg virus VP35 oligomerization domain Journal of Virology 2017 91 DOI:10.1128/jvi.01085-16 PMID:27847355 PMCID:PMC5215338
- Kirchdoerfer, R. N., Wasserman, H., Amarasinghe, G. K., Saphire, E. O. Filovirus structural biology: the molecules in the machine Current Topics in Microbiology and Immunology 2017 411:381-417 DOI:10.1007/82_2017_16 PMID:28795188
- Wec, A. Z., Nyakatura, E. K., Herbert, A. S., Howell, K. A., Holtsberg, F. W., Bakken, R. R., Mittler, E., Christin, J. R., Shulenin, S., Jangra, R. K., Bharrhan, S., Kuehne, A. I., et al. A "Trojan horse" bispecific-antibody strategy for broad protection against ebolaviruses Science 2016 354:350-354 DOI:10.1126/science.aag3267 PMID:27608667
- Goba, A., Khan, S. H., Fonnie, M., Fullah, M., Moigboi, A., Kovoma, A., Sinnah, V., Yoko, N., Rogers, H., Safai, S., Momoh, M., Koroma, V., et al. An outbreak of Ebola virus disease in the Lassa fever zone Journal of Infectious Diseases 2016 214:S110-S121 DOI:10.1093/infdis/jiw239 PMID:27402779 PMCID:PMC5050470
- Cross, R. W., Boisen, M. L., Millett, M. M., Nelson, D. S., Oottamasathien, D., Hartnett, J. N., Jones, A. B., Goba, A., Momoh, M., Fullah, M., Bornholdt, Z. A., Fusco, M. L., et al. Analytical validation of the ReEBOV antigen rapid test for point-of-care diagnosis of Ebola virus infection Journal of Infectious Diseases 2016 214:S210-S217 DOI:10.1093/infdis/jiw293 PMID:27587634 PMCID:PMC5050482
- Kirchdoerfer, R. N., Moyer, C. L., Abelson, D. M., Saphire, E. O. The Ebola virus VP30-NP interaction Is a regulator of viral RNA synthesis PLoS Pathogens 2016 12 DOI:10.1371/journal.ppat.1005937 PMID:27755595 PMCID:PMC5068707
- Saphire, E. O., Aman, M. J. Feverish quest for ebola immunotherapy: straight or cocktail? Trends in Microbiology 2016 24:684-686 DOI:10.1016/j.tim.2016.05.008 PMID:27338027
- Pallesen, J., Murin, C. D., de Val, N., Cottrell, C. A., Hastie, K. M., Turner, H. L., Fusco, M. L., Flyak, A. I., Zeitlin, L., Crowe, J. E., Andersen, K. G., Saphire, E. O., et al. Structures of Ebola virus GP and sGP in complex with therapeutic antibodies Nature Microbiology 2016 1 DOI:10.1038/nmicrobiol.2016.128 PMID:27562261 PMCID:PMC5003320
- Hastie, K. M., Igonet, S., Sullivan, B. M., Legrand, P., Zandonatti, M. A., Robinson, J. E., Garry, R. F., Rey, F. A., Oldstone, M. B., Saphire, E. O. Crystal structure of the prefusion surface glycoprotein of the prototypic arenavirus LCMV Nature Structural & Molecular Biology 2016 23:513-521 DOI:10.1038/nsmb.3210 PMID:27111888 PMCID:PMC4945123
- Howell, K. A., Qiu, X., Brannan, J. M., Bryan, C., Davidson, E., Holtsberg, F. W., Wec, A. Z., Shulenin, S., Biggins, J. E., Douglas, R., Enterlein, S. G., Turner, H. L., et al. Antibody treatment of Ebola and Sudan virus infection via a uniquely exposed epitope within the glycoprotein receptor-binding site Cell Reports 2016 15:1514-1526 DOI:10.1016/j.celrep.2016.04.026 PMID:27160900 PMCID:PMC4871745
- Hastie, K. M., Zandonatti, M., Liu, T., Li, S., Woods Jr., V. L., Saphire, E. O. Crystal structure of the oligomeric form of Lassa virus matrix protein Z Journal of Virology 2016 90:4556-4562 DOI:10.1128/jvi.02896-15 PMID:26912609 PMCID:PMC4836352
- Robinson, J. E., Hastie, K. M., Cross, R. W., Yenni, R. E., Elliott, D. H., Rouelle, J. A., Kannadka, C. B., Smira, A. A., Garry, C. E., Bradley, B. T., Yu, H., Shaffer, J. G., et al. Most neutralizing human monoclonal antibodies target novel epitopes requiring both Lassa virus glycoprotein subunits Nature Communications 2016 7 DOI:10.1038/ncomms11544 PMID:27161536 PMCID:PMC4866400
- Qiu, X., Audet, J., Lv, M., He, S., Wong, G., Wei, H., Luo, L., Fernando, L., Kroeker, A., Bovendo, H. F., Bello, A., Li, F., et al. Two-mAb cocktail protects macaques against the Makona variant of Ebola virus Science Translational Medicine 2016 8 DOI:10.1126/scitranslmed.aad9875 PMID:26962157
- Bornholdt, Z. A., Turner, H. L., Murin, C. D., Li, W., Sok, D., Souders, C. A., Piper, A. E., Goff, A., Shamblin, J. D., Wollen, S. E., Sprague, T. R., Fusco, M. L., et al. Isolation of potent neutralizing antibodies from a survivor of the 2014 Ebola virus outbreak Science 2016 351:1078-1083 DOI:10.1126/science.aad5788 PMID:26912366 PMCID:PMC4900763
- Oda, S., Noda, T., Wijesinghe, K. J., Halfmann, P., Bornholdt, Z. A., Abelson, D. M., Armbrust, T., Stahelin, R. V., Kawaoka, Y., Saphire, E. O. Crystal structure of Marburg virus VP40 reveals a broad, basic patch for matrix assembly and a requirement of the N-terminal domain for immunosuppression Journal of Virology 2016 90:1839-1848 DOI:10.1128/jvi.01597-15 PMID:26656687 PMCID:PMC4733994
- Flyak, A. I., Shen, X., Murin, C. D., Turner, H. L., David, J. A., Fusco, M. L., Lampley, R., Kose, N., Ilinykh, P. A., Kuzmina, N., Branchizio, A., King, H., et al. Cross-reactive and potent neutralizing antibody responses in human survivors of natural Ebolavirus infection Cell 2016 164:392-405 DOI:10.1016/j.cell.2015.12.022 PMID:26806128 PMCID:PMC4733404
- Bornholdt, Z. A., Ndungo, E., Fusco, M. L., Bale, S., Flyak, A. I., Crowe Jr., J. E., Chandran, K., Saphire, E. O. Host-primed Ebola virus GP exposes a hydrophobic NPC1 receptor-binding pocket, revealing a target for broadly neutralizing antibodies MBio 2016 7:e02154-15 DOI:10.1128/mBio.02154-15 PMID:26908579 PMCID:PMC4791852
- Holtsberg, F. W., Shulenin, S., Vu, H., Howell, K. A., Patel, S. J., Gunn, B., Karim, M., Lai, J. R., Frei, J. C., Nyakatura, E. K., Zeitlin, L., Douglas, R., et al. Pan-ebolavirus and pan-filovirus mouse monoclonal antibodies: protection against Ebola and Sudan viruses Journal of Virology 2016 90:266-278 DOI:10.1128/jvi.02171-15 PMID:26468533 PMCID:PMC4702560
- Kielian, M., Saphire, E. O. Potent antibody protection against an emerging alphavirus threat Cell 2015 163:1053-1054 DOI:10.1016/j.cell.2015.11.006 PMID:26590413
- Boisen, M. L., Oottamasathien, D., Jones, A. B., Millett, M. M., Nelson, D. S., Bornholdt, Z., Fusco, M. L., Abelson, D. M., Oda, S., Hartnett, J. N., Rowland, M. M., Heinrich, M. L., et al. Development of prototype filovirus recombinant antigen immunoassays Journal of Infectious Diseases 2015 212:S359-S367 DOI:10.1093/infdis/jiv353 PMID:26232440 PMCID:PMC4564556
- Clifton, M. C., Bruhn, J. F., Atkins, K., Webb, T. L., Baydo, R. O., Raymond, A., Lorimer, D. D., Edwards, T. E., Myler, P. J., Saphire, E. O. High-resolution crystal structure of dimeric VP40 from Sudan ebolavirus Journal of Infectious Diseases 2015 212:S167-S171 DOI:10.1093/infdis/jiv090 PMID:25957961 PMCID:PMC4564533
- Burton, D. R., Saphire, E. O. Swift antibodies to counter emerging viruses Proceedings of the National Academy of Sciences of the United States of America 2015 112:10082-10083 DOI:10.1073/pnas.1513050112 PMID:26261336 PMCID:PMC4547222
- Kirchdoerfer, R. N., Abelson, D. M., Li, S., Wood, M. R., Saphire, E. O. Assembly of the ebola virus nucleoprotein from a chaperoned VP35 complex Cell Reports 2015 12:140-149 DOI:10.1016/j.celrep.2015.06.003 PMID:26119732 PMCID:PMC4500542
- Fusco, M. L., Hashiguchi, T., Cassan, R., Biggins, J. E., Murin, C. D., Warfield, K. L., Li, S., Holtsberg, F. W., Shulenin, S., Vu, H., Olinger, G. G., Kim, D. H., et al. Protective mAbs and cross-reactive mAbs raised by immunization with engineered Marburg virus GPs PLoS Pathogens 2015 11:e1005016 DOI:10.1371/journal.ppat.1005016 PMID:26115029 PMCID:PMC4482612
- Saphire, E. O. New advances in the effort against Ebola Cell Host & Microbe 2015 17:545-547 DOI:10.1016/j.chom.2015.04.015 PMID:25974296
- Flyak, A. I., Ilinykh, P. A., Murin, C. D., Garron, T., Shen, X., Fusco, M. L., Hashiguchi, T., Bornholdt, Z. A., Slaughter, J. C., Sapparapu, G., Klages, C., Ksiazek, T. G., et al. Mechanism of human antibody-mediated neutralization of Marburg virus Cell 2015 160:893-903 DOI:10.1016/j.cell.2015.01.031 PMID:25723164 PMCID:PMC4344968
- Hashiguchi, T., Fusco, M. L., Bornholdt, Z. A., Lee, J. E., Flyak, A. I., Matsuoka, R., Kohda, D., Yanagi, Y., Hammel, M., Crowe Jr., J. E., Saphire, E. O. Structural basis for Marburg virus neutralization by a cross-reactive human antibody Cell 2015 160:904-912 DOI:10.1016/j.cell.2015.01.041 PMID:25723165 PMCID:PMC4344967
- Boisen, M. L., Schieffelin, J. S., Goba, A., Oottamasathien, D., Jones, A. B., Shaffer, J. G., Hastie, K. M., Hartnett, J. N., Momoh, M., Fullah, M., Gabiki, M., Safa, S., et al. Multiple circulating infections can mimic the early stages of viral hemorrhagic fevers and possible human exposure to filoviruses in Sierra Leone prior to the 2014 outbreak Viral Immunology 2015 28:19-31 DOI:10.1089/vim.2014.0108 PMID:25531344 PMCID:PMC4287116
- Murin, C. D., Fusco, M. L., Bornholdt, Z. A., Qiu, X., Olinger, G. G., Zeitlin, L., Kobinger, G. P., Ward, A. B., Saphire, E. O. Structures of protective antibodies reveal sites of vulnerability on Ebola virus Proceedings of the National Academy of Sciences of the United States of America 2014 111:17182-17187 DOI:10.1073/pnas.1414164111 PMID:25404321 PMCID:PMC4260551
- Kuhn, J. H., Andersen, K. G., Bao, Y., Bavari, S., Becker, S., Bennett, R. S., Bergman, N. H., Blinkova, O., Bradfute, S., Brister, J. R., Bukreyev, A., Chandran, K., et al. Filovirus RefSeq entries: evaluation and selection of filovirus type variants, type sequences, and names Viruses-Basel 2014 6:3663-3682 DOI:10.3390/v6093663 PMID:25256396 PMCID:PMC4189044
- Zhang, A. P. P., Bornholdt, Z. A., Abelson, D. M., Saphire, E. O. Crystal structure of Marburg virus VP24 Journal of Virology 2014 88:5859-5863 DOI:10.1128/jvi.03565-13 PMID:24574400 PMCID:PMC4019143
- Kuhn, J. H., Bao, Y., Bavari, S., Becker, S., Bradfute, S., Brauburger, K., Brister, J. R., Bukreyev, A. A., Cai, Y., Chandran, K., Davey, R. A., Dolnik, O., et al. Virus nomenclature below the species level: a standardized nomenclature for filovirus strains and variants rescued from cDNA Archives of Virology 2014 159:1229-1237 DOI:10.1007/s00705-013-1877-2 PMID:24190508 PMCID:PMC4010566
- Bukreyev, A. A., Chandran, K., Dolnik, O., Dye, J. M., Ebihara, H., Leroy, E. M., Muhlberger, E., Netesov, S. V., Patterson, J. L., Paweska, J. T., Saphire, E. O., Smither, S. J., et al. Discussions and decisions of the 2012-2014 International Committee on Taxonomy of Viruses (ICTV) Filoviridae Study Group, January 2012-June 2013 Archives of Virology 2014 159:821-830 DOI:10.1007/s00705-013-1846-9 PMID:24122154 PMCID:PMC3984924
- Clifton, M. C., Kirchdoerfer, R. N., Atkins, K., Abendroth, J., Raymond, A., Grice, R., Barnes, S., Moen, S., Lorimer, D., Edwards, T. E., Myler, P. J., Saphire, E. O. Structure of the Reston ebolavirus VP30 C-terminal domain Acta Crystallographica Section F-Structural Biology Communications 2014 70:457-460 DOI:10.1107/s2053230x14003811 PMID:24699737 PMCID:PMC3976061
- Diebolder, C. A., Beurskens, F. J., de Jong, R. N., Koning, R. I., Strumane, K., Lindorfer, M. A., Voorhorst, M., Ugurlar, D., Rosati, S., Heck, A. J. R., van de Winkel, J. G. J., Wilson, I. A., et al. Complement is activated by IgG hexamers assembled at the cell surface Science 2014 343:1260-1263 DOI:10.1126/science.1248943 PMID:24626930 PMCID:PMC4250092
- Bruhn, J. F., Barnett, K. C., Bibby, J., Thomas, J. M. H., Keegan, R. M., Rigden, D. J., Bornholdt, Z. A., Saphire, E. O. Crystal structure of the Nipah virus phosphoprotein tetramerization domain Journal of Virology 2014 88:758-762 DOI:10.1128/jvi.02294-13 PMID:24155387 PMCID:PMC3911761
- Shaffer, J. G., Grant, D. S., Schieffelin, J. S., Boisen, M. L., Goba, A., Hartnett, J. N., Levy, D. C., Yenni, R. E., Moses, L. M., Fullah, M., Momoh, M., Fonnie, M., et al. Lassa Fever in post-conflict Sierra Leone PLoS Neglected Tropical Diseases 2014 8:e2748 DOI:10.1371/journal.pntd.0002748 PMID:24651047 PMCID:PMC3961205
- Olal, D., Dick, A., Woods Jr., V. L., Liu, T., Li, S., Devignot, S., Weber, F., Saphire, E. O., Daumke, O. Structural insights into RNA encapsidation and helical assembly of the Toscana virus nucleoprotein Nucleic Acids Research 2014 42:6025-6037 DOI:10.1093/nar/gku229 PMID:24688060 PMCID:PMC4027202
- West, B. R., Hastie, K. M., Saphire, E. O. Structure of the LCMV nucleoprotein provides a template for understanding arenavirus replication and immunosuppression Acta Crystallographica Section D-Biological Crystallography 2014 70:1764-1769 DOI:10.1107/s1399004714007883 PMID:24914986 PMCID:PMC4051510
- Saphire, E. O. An update on the use of antibodies against the filoviruses Immunotherapy 2013 5:1221-1233 DOI:10.2217/imt.13.124 PMID:24188676 PMCID:PMC4465755
- Bale, S., Julien, J. P., Bornholdt, Z. A., Krois, A. S., Wilson, I. A., Saphire, E. O. Ebolavirus VP35 Coats the Backbone of Double-Stranded RNA for Interferon Antagonism Journal of Virology 2013 87:10385-10388 DOI:10.1128/jvi.01452-13 PMID:23824825 PMCID:PMC3753998
- Bornholdt, Z. A., Noda, T., Abelson, D. M., Halfmann, P., Wood, M. R., Kawaoka, Y., Saphire, E. O. Structural rearrangement of ebola virus VP40 begets multiple functions in the virus life cycle Cell 2013 154:763-774 DOI:10.1016/j.cell.2013.07.015 PMID:23953110 PMCID:PMC4138722
- Kuhn, J. H., Bao, Y. M., Bavari, S., Becker, S., Bradfute, S., Brister, J. R., Bukreyev, A. A., Chandran, K., Davey, R. A., Dolnik, O., Dye, J. M., Enterlein, S., et al. Virus nomenclature below the species level: a standardized nomenclature for natural variants of viruses assigned to the family Filoviridae Archives of Virology 2013 158:301-311 DOI:10.1007/s00705-012-1454-0 PMID:23001720 PMCID:PMC3535543
- Koellhoffer, J. F., Chen, G., Sandesara, R. G., Bale, S., Saphire, E. O., Chandran, K., Sidhu, S. S., Lai, J. R. Two synthetic antibodies that recognize and neutralize distinct proteolytic forms of the ebola virus envelope glycoprotein ChemBioChem 2012 13:2549-2557 DOI:10.1002/cbic.201200493 PMID:23111988 PMCID:PMC3684266
- Bale, S., Julien, J. P., Bornholdt, Z. A., Kimberlin, C. R., Halfmann, P., Zandonatti, M. A., Kunert, J., Kroon, G. J. A., Kawaoka, Y., MacRae, I. J., Wilson, I. A., Saphire, E. O. Marburg virus VP35 can both fully coat the backbone and cap the ends of dsRNA for interferon antagonism PLoS Pathogens 2012 8:e1002916 DOI:10.1371/journal.ppat.1002916 PMID:23028316 PMCID:PMC3441732
- Hastie, K. M., King, L. B., Zandonatti, M. A., Saphire, E. O. Structural basis for the dsrna specificity of the lassa virus np exonuclease PLoS One 2012 7:e44211 DOI:10.1371/journal.pone.0044211 PMID:22937163 PMCID:PMC3429428
- Zhang, A. P. P., Abelson, D. M., Bornholdt, Z., Liu, T., Woods, V. L., Saphire, E. O. The ebolavirus VP24 interferon antagonist: know your enemy Virulence 2012 3:440-445 DOI:10.4161/viru.21302 PMID:23076242 PMCID:PMC3485981
- Hastie, K. M., Bale, S., Kimberlin, C. R., Saphire, E. O. Hiding the evidence: two strategies for innate immune evasion by hemorrhagic fever viruses Current Opinion in Virology 2012 2:151-156 DOI:10.1016/j.coviro.2012.01.003 PMID:22482712 PMCID:PMC3758253
- Olal, D., Kuehne, A. I., Bale, S., Halfmann, P., Hashiguchi, T., Fusco, M. L., Lee, J. E., King, L. B., Kawaoka, Y., Dye, J. M., Saphire, E. O. Structure of an antibody in complex with its mucin domain linear epitope that is protective against ebola virus Journal of Virology 2012 86:2809-2816 DOI:10.1128/jvi.05549-11 PMID:22171276 PMCID:PMC3302272
- Zhang, A. P. P., Bornholdt, Z. A., Liu, T., Abelson, D. M., Lee, D. E., Li, S., Woods, V. L., Saphire, E. O. The ebola virus interferon antagonist vp24 directly binds stat1 and has a novel, pyramidal fold PLoS Pathogens 2012 8:e1002550 DOI:10.1371/journal.ppat.1002550 PMID:22383882 PMCID:PMC3285596
- Brecher, M., Schornberg, K. L., Delos, S. E., Fusco, M. L., Saphire, E. O., White, J. M. Cathepsin cleavage potentiates the ebola virus glycoprotein to undergo a subsequent fusion-relevant conformational change Journal of Virology 2012 86:364-372 DOI:10.1128/jvi.05708-11 PMID:22031933 PMCID:PMC3255896
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- Hastie, K. M., Liu, T., Li, S., King, L. B., Ngo, N., Zandonatti, M. A., Woods, V. L., de la Torre, J. C., Saphire, E. O. Crystal structure of the lassa virus nucleoprotein-RNA complex reveals a gating mechanism for RNA binding Proceedings of the National Academy of Sciences of the United States of America 2011 108:19365-19370 DOI:10.1073/pnas.1108515108 PMID:22084115 PMCID:PMC3228486
- Bale, S., Liu, T., Li, S., Wang, Y., Abelson, D., Fusco, M., Woods, V. L., Saphire, E. O. Ebola virus glycoprotein needs an additional trigger, beyond proteolytic priming for membrane fusion PLoS Neglected Tropical Diseases 2011 5:e1395 DOI:10.1371/journal.pntd.0001395 PMID:22102923 PMCID:PMC3216919
- Hastie, K. M., Kimberlin, C. R., Zandonatti, M. A., MacRae, I. J., Saphire, E. O. Structure of the Lassa virus nucleoprotein reveals a dsRNA-specific 3' to 5' exonuclease activity essential for immune suppression Proceedings of the National Academy of Sciences of the United States of America 2011 108:2396-2401 DOI:10.1073/pnas.1016404108 PMID:21262835 PMCID:PMC3038715
- Kimberlin, C. R., Bornholdt, Z. A., Li, S., Woods, V. L., MacRae, I. J., Saphire, E. O. Ebolavirus VP35 uses a bimodal strategy to bind dsRNA for innate immune suppression Proceedings of the National Academy of Sciences of the United States of America 2010 107:314-319 DOI:10.1073/pnas.0910547107 PMID:20018665 PMCID:PMC2806767
- Lee, J. E., Saphire, E. O. Ebolavirus glycoprotein structure and mechanism of entry Future Virology 2009 4:621-635 DOI:10.2217/fvl.09.56 PMID:20198110 PMCID:PMC2829775
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- Lee, J. E., Saphire, E. O. Neutralizing ebolavirus: Structural insights into the envelope glycoprotein and antibodies targeted against it Current Opinion in Structural Biology 2009 19:408-417 DOI:10.1016/j.sbi.2009.05.004 PMID:19559599 PMCID:PMC2759674
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Saphire, E. O., Montero, M., Menendez, A., van Houten, N. E., Irving, M. B., Pantophlet, R., Zwick, M. B., Parren, Pwhi, Burton, D. R., Scott, J. K., Wilson, I. A. Structure of a high-affinity "mimotope" peptide bound to HIV-1-neutralizing antibody b12 explains its inability to elicit gp120 cross-reactive antibodies Journal of Molecular Biology 2007 369:696-709 DOI:10.1016/j.jmb.2007.01.060 PMID:17445828 PMCID:PMC1995417
- Cardenas, W. B., Loo, Y. M., Gale, M., Hartman, A. L., Kimberlin, C. R., Martinez-Sobrido, L., Saphire, E. O., Basler, C. F. Ebola virus VP35 protein binds double-stranded RNA and inhibits alpha/beta interferon production induced by RIG-I signaling Journal of Virology 2006 80:5168-5178 DOI:10.1128/jvi.02199-05 PMID:16698997
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Zwick, M. B., Saphire, E. O., Burton, D. R. gp41: HIV's shy protein Nature Medicine 2004 10:133-134 DOI:10.1038/nm0204-133 PMID:14760422
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Stanfield, R. L., Ghiara, J. B., Saphire, E. M., Profy, A. T., Wilson, I. A. Recurring conformation of the human immunodeficiency virus type 1 gp120 V3 loop Virology 2003 315:159-173 DOI:10.1016/s0042-6822(03)00525-7 PMID:14592768
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Zwick, M. B., Parren, P. W., Saphire, E. O., Church, S., Wang, M., Scott, J. K., Dawson, P. E., Wilson, I. A., Burton, D. R. Molecular features of the broadly neutralizing immunoglobulin G1 b12 required for recognition of human immunodeficiency virus type 1 gp120 Journal of Virology 2003 77:5863-5876 DOI:10.1128/jvi.77.10.5863-5876.2003 PMID:12719580 PMCID:PMC154005
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Saphire, E. O., Stanfield, R. L., Crispin, M. D. M., Morris, G., Zwick, M. B., Pantophlet, R. A., Parren, P. W. H. I., Rudd, P. M., Dwek, R. A., Burton, D. R., Wilson, I. A. Crystal structure of an intact human IgG: antibody asymmetry, flexibility, and a guide for HIV-1 vaccine design Advances in Experimental Medicine and Biology 2003 535:55-66 DOI:10.1007/978-1-4615-0065-0_4 PMID:14714888
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Pantophlet, R., Ollmann Saphire, E., Poignard, P., Parren, P. W., Wilson, I. A., Burton, D. R. Fine mapping of the interaction of neutralizing and nonneutralizing monoclonal antibodies with the CD4 binding site of human immunodeficiency virus type 1 gp120 Journal of Virology 2003 77:642-658 DOI:10.1128/jvi.77.1.642-658.2003 PMID:12477867 PMCID:PMC140633
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Scanlan, C. N., Pantophlet, R., Wormald, M. R., Saphire, E. O., Calarese, D., Stanfield, R., Wilson, I. A., Katinger, H., Dwek, R. A., Burton, D. R., Rudd, P. M. The carbohydrate epitope of the neutralizing anti-HIV-1 antibody 2G12 Advances in Experimental Medicine and Biology 2003 535:205-218 DOI:10.1007/978-1-4615-0065-0_13 PMID:14714897
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Scanlan, C. N., Pantophlet, R., Wormald, M. R., Saphire, E. O., Stanfield, R., Wilson, I. A., Katinger, H., Dwek, R. A., Rudd, P. M., Burton, D. R. The broadly neutralizing anti-human immunodeficiency virus type 1 antibody 2g12 recognizes a cluster of α1→2 mannose residues on the outer face of gp120 Journal of Virology 2002 76:7306-7321 DOI:10.1128/jvi.76.14.7306-7321.2002 PMID:12072529 PMCID:PMC136327
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Saphire, E. O., Stanfield, R. L., Crispin, M. D. M., Parren, P. W. H. I., Rudd, P. M., Dwek, R. A., Burton, D. R., Wilson, I. A. Contrasting IgG structures reveal extreme asymmetry and flexibility Journal of Molecular Biology 2002 319:9-18 DOI:10.1016/s0022-2836(02)00244-9 PMID:12051932
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Saphire, E. O. Structure-based drug design IDrugs 2002 5:658-661 PMID:14686344
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Zwick, M. B., Labrijn, A. F., Wang, M., Spenlehauer, C., Saphire, E. O., Binley, J. M., Moore, J. P., Stiegler, G., Katinger, H., Burton, D. R., Parren, P. W. Broadly neutralizing antibodies targeted to the membrane-proximal external region of human immunodeficiency virus type 1 glycoprotein gp41 Journal of Virology 2001 75:10892-10905 DOI:10.1128/jvi.75.22.10892-10905.2001 PMID:11602729 PMCID:PMC114669
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Saphire, E. O., Parren, P. W. H. I. Listening for viral infection Nature Biotechnology 2001 19:823-824 DOI:10.1038/nbt0901-823 PMID:11533636
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Saphire, E. O., Parren, P. W. H. I., Pantophlet, R., Zwick, M. B., Morris, G. M., Rudd, P. M., Dwek, R. A., Stanfield, R. L., Burton, D. R., Wilson, I. A. Crystal structure of a neutralizing human IGG against HIV-1: a template for vaccine design Science 2001 293:1155-1159 DOI:10.1126/science.1061692 PMID:11498595
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Burton, D. R., Saphire, E. O., Parren, P. W. H. I. A model for neutralization of viruses based on antibody coating of the virion surface Current Topics in Microbiology and Immunology 2001 260:109-143 PMID:11443871
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Saphire, E. O., Parren, P. W., Barbas, C. F., 3rd, Burton, D. R., Wilson, I. A. Crystallization and preliminary structure determination of an intact human immunoglobulin, b12: An antibody that broadly neutralizes primary isolates of HIV-1 Acta Crystallographica Section D-Biological Crystallography 2001 57:168-171 DOI:10.1107/s0907444900017376 PMID:11134947
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Poignard, P., Saphire, E. O., Parren, P. W. H. I., Burton, D. R. Gp120: Biologic aspects of structural features Annual Review of Immunology 2001 19:253-274 DOI:10.1146/annurev.immunol.19.1.253 PMID:11244037
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chapter
- Wasserman, H., Saphire, E. O. More than meets the eye: hidden structures in the proteome Annual Review of Virology. 373-386. 2016 DOI:10.1146/annurev-virology-100114-054923