recent publications
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academic article
- Sheu-Gruttadauria, J., Xiao, Y., Gebert, L. F. R., Macrae, I. J. Beyond the seed: structural basis for supplementary microRNA targeting by human Argonaute2 EMBO Journal 2019 38 DOI:10.15252/embj.2018101153 PMID:31268608 PMCID:PMC6600645
- Sheu-Gruttadauria, J., Pawlica, P., Klum, S. M., Wang, S., Yario, T. A., Oakdale, N. T. S., Steitz, J. A., Macrae, I. J. Structural basis for target-directed microRNA degradation Molecular Cell 2019 75:1243-1255 DOI:10.1016/j.molcel.2019.06.019 PMID:31353209 PMCID:PMC6754277
- Foss, D. V., Schirle, N. T., Macrae, I. J., Pezacki, J. P. Structural insights into interactions between viral suppressor of RNA silencing protein p19 mutants and small RNAs FEBS Open Bio 2019 9:1042-1051 DOI:10.1002/2211-5463.12644 PMID:31021526 PMCID:PMC6551489
- Xiao, Y., Macrae, I. J. Toward a comprehensive view of microRNA biology Molecular Cell 2019 75:666-668 DOI:10.1016/j.molcel.2019.08.001 PMID:31442421
- Chahal, J., Gebert, L. F. R., Gan, H. H., Camacho, E., Gunsalus, K. C., Macrae, I. J., Sagan, S. M. miR-122 and Ago interactions with the HCV genome alter the structure of the viral 5 terminus Nucleic Acids Research 2019 47:5307-5324 DOI:10.1093/nar/gkz194 PMID:30941417 PMCID:PMC6547439
- Ziv, O., Gabryelska, M. M., Lun, A. T. L., Gebert, L. F. R., Sheu-Gruttadauria, J., Meredith, L. W., Liu, Z. Y., Kwok, C. K., Qin, C. F., Macrae, I. J., Goodfellow, I., Marioni, J. C., et al. COMRADES determines in vivo RNA structures and interactions Nature Methods 2018 15:785-788 DOI:10.1038/s41592-018-0121-0 PMID:30202058 PMCID:PMC6168409
- Klum, S. M., Chandradoss, S. D., Schirle, N. T., Joo, C., Macrae, I. J. Helix-7 in Argonaute2 shapes the microRNA seed region for rapid target recognition EMBO Journal 2018 37:75-88 DOI:10.15252/embj.201796474 PMID:28939659 PMCID:PMC5753032
- Sheu-Gruttadauria, J., Macrae, I. J. Phase transitions in the assembly and function of human miRISC Cell 2018 173:946-957 DOI:10.1016/j.cell.2018.02.051 PMID:29576456 PMCID:PMC5935535
- Sheu-Gruttadauria, J., MacRae, I. J. Structural foundations of RNA silencing by Argonaute Journal of Molecular Biology 2017 429:2619-2639 DOI:10.1016/j.jmb.2017.07.018 PMID:28757069 PMCID:PMC5576611
- Wu, M. K., de Kock, L., Conwell, L. S., Stewart, C. J. R., King, B. R., Choong, C. S., Hussain, K., Sabbaghian, N., Macrae, I. J., Fabian, M. R., Foulkes, W. D. Functional characterization of multiple DICER1 mutations in an adolescent Endocrine-Related Cancer 2016 23:L1-L5 DOI:10.1530/erc-15-0460 PMID:26545620
- Schirle, N. T., Kinberger, G. A., Murray, H. F., Lima, W. F., Prakash, T. P., Macrae, I. J. Structural analysis of human Argonaute-2 bound to a modified siRNA guide Journal of the American Chemical Society 2016 138:8694-8697 DOI:10.1021/jacs.6b04454 PMID:27380263 PMCID:PMC4993527
- Suter, S. R., Sheu-Gruttadauria, J., Schirle, N. T., Valenzuela, R., Ball-Jones, A. A., Onizuka, K., Macrae, I. J., Beal, P. A. Structure-guided control of siRNA off-target effects Journal of the American Chemical Society 2016 138:8667-8669 DOI:10.1021/jacs.6b06137 PMID:27387838 PMCID:PMC4962782
- Schirle, N. T., Sheu-Gruttadauria, J., Chandradoss, S. D., Joo, C., Macrae, I. J. Water-mediated recognition of t1-adenosine anchors Argonaute2 to microRNA targets Elife 2015 4 DOI:10.7554/eLife.07646 PMID:26359634 PMCID:PMC4606517
- Chandradoss, S. D., Schirle, N. T., Szczepaniak, M., Macrae, I. J., Joo, C. A dynamic search process underlies microRNA targeting Cell 2015 162:96-107 DOI:10.1016/j.cell.2015.06.032 PMID:26140593 PMCID:PMC4768356
- Schirle, N. T., Sheu-Gruttadauria, J., Macrae, I. J. Structural basis for microRNA targeting Science 2014 346:608-613 DOI:10.1126/science.1258040 PMID:25359968 PMCID:PMC4313529
- Horman, S. R., Janas, M. M., Litterst, C., Wang, B., Macrae, I. J., Sever, M. J., Morrissey, D. V., Graves, P., Luo, B., Umesalma, S., Qi, H. H., Miraglia, L. J., et al. Akt-mediated phosphorylation of argonaute 2 downregulates cleavage and upregulates translational repression of MicroRNA targets Molecular Cell 2013 50:356-367 DOI:10.1016/j.molce1.2013.03.015 PMID:23603119 PMCID:PMC3654076
- Xiong, X. P., Kurthkoti, K., Chang, K. Y., Lichinchi, G., De, N., Schneemann, A., Macrae, I. J., Rana, T. M., Perrimon, N., Zhou, R. Core small nuclear ribonucleoprotein particle splicing factor SmD1 modulates RNA interference in Drosophila Proceedings of the National Academy of Sciences of the United States of America 2013 110:16520-16525 DOI:10.1073/pnas.1315803110 PMID:24067655 PMCID:PMC3799365
- De, N., Young, L., Lau, P. W., Meisner, N. C., Morrissey, D. V., Macrae, I. J. Highly complementary target RNAs promote release of guide RNAs from human Argonaute2 Molecular Cell 2013 50:344-355 DOI:10.1016/j.molcel.2013.04.001 PMID:23664376 PMCID:PMC3746828
- Lau, P. W., Potter, C. S., Carragher, B., MacRae, I. J. DOLORS: versatile strategy for internal labeling and domain localization in electron microscopy Structure 2012 20:1995-2002 DOI:10.1016/j.str.2012.10.019 PMID:23217681 PMCID:PMC3535077
- Bale, S., Julien, J. P., Bornholdt, Z. A., Kimberlin, C. R., Halfmann, P., Zandonatti, M. A., Kunert, J., Kroon, G. J. A., Kawaoka, Y., MacRae, I. J., Wilson, I. A., Saphire, E. O. Marburg virus VP35 can both fully coat the backbone and cap the ends of dsRNA for interferon antagonism PLoS Pathogens 2012 8:e1002916 DOI:10.1371/journal.ppat.1002916 PMID:23028316 PMCID:PMC3441732
- Pratt, A. J., Rambo, R. P., Lau, P. W., MacRae, I. J. Preparation and characterization of the extracellular domain of human Sid-1 PLoS One 2012 7:e33607 DOI:10.1371/journal.pone.0033607 PMID:22509261 PMCID:PMC3324469
- Guiley, K. Z.*, Pratt, A. J.*, MacRae, I. J. Single-pot enzymatic synthesis of Dicer-substrate siRNAs Nucleic Acids Research 2012 40:e40 DOI:10.1093/nar/gkr1174 PMID:22189103 PMCID:PMC3299999 (* = equal contribution)
- Schirle, N. T., MacRae, I. J. The crystal structure of human Argonaute2 Science 2012 336:1037-1040 DOI:10.1126/science.1221551 PMID:22539551 PMCID:PMC3521581
- Lau, P. W., Guiley, K. Z., De, N., Potter, C. S., Carragher, B., MacRae, I. J. The molecular architecture of human Dicer Nature Structural & Molecular Biology 2012 19:436-440 DOI:10.1038/nsmb.2268 PMID:22426548 PMCID:PMC3319852
- De, N., Macrae, I. J. Purification and assembly of human Argonaute, Dicer, and TRBP complexes Methods in Molecular Biology 2011 725:107-119 DOI:10.1007/978-1-61779-046-1_8 PMID:21528450 PMCID:PMC3532949
- Hastie, K. M., Kimberlin, C. R., Zandonatti, M. A., MacRae, I. J., Saphire, E. O. Structure of the Lassa virus nucleoprotein reveals a dsRNA-specific 3' to 5' exonuclease activity essential for immune suppression Proceedings of the National Academy of Sciences of the United States of America 2011 108:2396-2401 DOI:10.1073/pnas.1016404108 PMID:21262835 PMCID:PMC3038715
- Kimberlin, C. R., Bornholdt, Z. A., Li, S., Woods, V. L., MacRae, I. J., Saphire, E. O. Ebolavirus VP35 uses a bimodal strategy to bind dsRNA for innate immune suppression Proceedings of the National Academy of Sciences of the United States of America 2010 107:314-319 DOI:10.1073/pnas.0910547107 PMID:20018665 PMCID:PMC2806767
- Lau, P. W., Potter, C. S., Carragher, B., MacRae, I. J. Structure of the human Dicer-TRBP complex by electron microscopy Structure 2009 17:1326-1332 DOI:10.1016/j.str.2009.08.013 PMID:19836333 PMCID:PMC2880462
- Pratt, A. J., MacRae, I. J. The RNA-induced silencing complex: A versatile gene-silencing machine Journal of Biological Chemistry 2009 284:17897-17901 DOI:10.1074/jbc.R900012200 PMID:19342379 PMCID:PMC2709356
- Lau, P. W., MacRae, I. J. The molecular machines that mediate microRNA maturation Journal of Cellular and Molecular Medicine 2009 13:54-60 DOI:10.1111/j.1582-4934.2008.00520.x PMID:19175700 PMCID:PMC3823036
- Ma, E., MacRae, I. J., Kirsch, J. F., Doudna, J. A. Autoinhibition of human dicer by its internal helicase domain Journal of Molecular Biology 2008 380:237-243 DOI:10.1016/j.jmb.2008.05.005 PMID:18508075 PMCID:PMC2927216
- MacRae, I. J., Ma, E., Zhou, M., Robinson, C. V., Doudna, J. A. In vitro reconstitution of the human RISC-loading complex Proceedings of the National Academy of Sciences of the United States of America 2008 105:512-517 DOI:10.1073/pnas.0710869105 PMID:18178619 PMCID:PMC2206567
- MacRae, I. J., Doudna, J. A. An unusual case of pseudo-merohedral twinning in orthorhombic crystals of Dicer Acta Crystallographica Section D-Biological Crystallography 2007 63:993-999 DOI:10.1107/s0907444907036128 PMID:17704568
- MacRae, I. J., Doudna, J. A. Ribonuclease revisited: structural insights into ribonuclease III family enzymes Current Opinion in Structural Biology 2007 17:138-145 DOI:10.1016/j.sbi.2006.12.002 PMID:17194582
- MacRae, I. J., Zhou, K., Doudna, J. A. Structural determinants of RNA recognition and cleavage by Dicer Nature Structural & Molecular Biology 2007 14:934-940 DOI:10.1038/nsmb1293 PMID:17873886
- MacRae, I. J., Zhou, K. H., Li, F., Repic, A., Brooks, A. N., Cande, W. Z., Adams, P. D., Doudna, J. A. Structural basis for double-stranded RNA processing by Dicer Science 2006 311:195-198 DOI:10.1126/science.1121638 PMID:16410517
- Macrae, I. J., Li, F., Zhou, K., Cande, W. Z., Doudna, J. A. Structure of Dicer and mechanistic implications for RNAi Cold Spring Harbor Symposia on Quantitative Biology 2006 71:73-80 DOI:10.1101/sqb.2006.71.042 PMID:17381283
- Hanna, E., Ng, K. F., MacRae, I. J., Bley, C. J., Fisher, A. J., Segel, I. H. Kinetic and stability properties of Penicillium chrysogenum ATP sulfurylase missing the C-terminal regulatory domain Journal of Biological Chemistry 2004 279:4415-4424 DOI:10.1074/jbc.M311317200 PMID:14613928
- Hanna, E., MacRae, I. J., Medina, D. C., Fisher, A. J., Segel, I. H. ATP sulfurylase from the hyperthermophilic chemolithotroph Aquifex aeolicus Archives of Biochemistry and Biophysics 2002 406:275-288 DOI:10.1016/s0003-9861(02)00428-9 PMID:12361716
- MacRae, I. J., Segel, I. H., Fisher, A. J. Allosteric inhibition via R-state destabilization in ATP sulfurylase from Penicillium chrysogenum Nature Structural Biology 2002 9:945-949 DOI:10.1038/nsb868 PMID:12426581
- Beynon, J. D., MacRae, I. J., Huston, S. L., Nelson, D. C., Segel, I. H., Fisher, A. J. Crystal structure of ATP sulfurylase from the bacterial symbiont of the hydrothermal vent tubeworm Riftia pachyptila Biochemistry 2001 40:14509-14517 DOI:10.1021/bi0156431 PMID:11724564
- MacRae, I. J., Segel, I. H., Fisher, A. J. Crystal structure of atp sulfurylase from penicillium chrysogenum: Insights into the allosteric regulation of sulfate assimilation Biochemistry 2001 40:6795-6804 DOI:10.1021/bi010367w PMID:11389593
- Medina, D. C., Hanna, E., MacRae, I. J., Fisher, A. J., Segel, I. H. Temperature effects on the allosteric transition of atp sulfurylase from penicillium chrysogenum Archives of Biochemistry and Biophysics 2001 393:51-60 DOI:10.1006/abbi.2001.2490 PMID:11516160
- MacRae, I. J., Segel, I. H., Fisher, A. J. Crystal structure of adenosine 5 '-phosphosulfate kinase from Penicillium chrysogenum Biochemistry 2000 39:1613-1621 DOI:10.1021/bi9924157 PMID:10677210
- MacRae, I. J., Hanna, E., Ho, J. D., Fisher, A. J., Segel, I. H. Induction of positive cooperativity by amino acid replacements within the C-terminal domain of penicillium chrysogenum ATP sulfurylase Journal of Biological Chemistry 2000 275:36303-36310 DOI:10.1074/jbc.M005992200 PMID:10956658
- MacRae, I. J., Segel, I. H. Adenosine 5 '-phosphosulfate (APS) kinase: Diagnosing the mechanism of substrate inhibition Archives of Biochemistry and Biophysics 1999 361:277-282 DOI:10.1006/abbi.1998.0993 PMID:9882457
- MacRae, I. J., Rose, A. B., Segel, I. H. Adenosine 5 '-phosphosulfate kinase from Penicillium chrysogenum - site-directed mutagenesis at putative phosphoryl-accepting and ATP P-loop residues Journal of Biological Chemistry 1998 273:28583-28589 DOI:10.1074/jbc.273.44.28583 PMID:9786849
- MacRae, I., Segel, I. H. ATP sulfurylase from filamentous fungi: which sulfonucleotide is the true allosteric effector? Archives of Biochemistry and Biophysics 1997 337:17-26 DOI:10.1006/abbi.1996.9751 PMID:8990263
