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Cell-Free System

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  • A 5' leader of Rbm3, a cold stress-induced mRNA, mediates internal initiation of translation with increased efficiency under conditions of mild hypothermia  Academic Article
  • A Rab GTPase is required for homotypic assembly of the endoplasmic reticulum  Academic Article
  • A cell free system to study reassembly of the nuclear-envelope at the end of mitosis  Academic Article
  • A cell-free biochemical complementation assay reveals complex and redundant cytosolic requirements for lrp endocytosis  Academic Article
  • A cell-free expression and purification process for rapid production of protein biologics  Academic Article
  • A fractionated cell-free system for analysis of prophase nuclear disassembly  Academic Article
  • A novel prefusion complex formed during protein-transport between golgi cisternae in a cell-free system  Academic Article
  • A recellularized human colon model identifies cancer driver genes  Academic Article
  • Activity of yeast extracts in cell-free stimulation of DNA-replication  Academic Article
  • Alteration of bacillus-subtilis glutamine-synthetase results in overproduction of enzyme  Academic Article
  • An enzymatic function associated with transformation of fibroblasts by oncogenic viruses. I. Chick embryo fibroblast cultures transformed by avian RNA tumor viruses  Academic Article
  • An enzymatic function associated with transformation of fibroblasts by oncogenic viruses. II. Mammalian fibroblast cultures transformed by DNA and RNA tumor viruses  Academic Article
  • Analysis of RNA polymerase III transcription in vitro using chromatin and cloned gene templates  Academic Article
  • Analysis of RNA processing reactions using cell free systems: 3' end cleavage of pre-mRNA substrates in vitro  Academic Article
  • Antisense masking reveals contributions of mrna- rrna base pairing to translation of gtx and fgf2 mrnas  Academic Article
  • Asia growth in membrane protein structure  Academic Article
  • Bacterial lipopolysaccharide and inflammatory mediators augment IL-6 secretion by human endothelial cells  Academic Article
  • Birth of a prion: Spontaneous generation revisited  Academic Article
  • Cell-free expression of disulfide-containing eukaryotic proteins for structural biology  Academic Article
  • Cell-free expression of functional receptor tyrosine kinases  Academic Article
  • Cell-free production of a functional oligomeric form of a Chlamydia major outer-membrane protein (MOMP) for vaccine development  Academic Article
  • Cell-free production of biologically active polypeptides: application to the synthesis of antibacterial peptide cecropin  Academic Article
  • Cell-free synthesis and segregation of beta-2-microglobulin  Academic Article
  • Characterization of a novel iodocyanopindolol and sm-11044 binding protein, which may mediate relaxation of depolarized rat colon tonus  Academic Article
  • Characterization of protein-transport between successive compartments of the golgi-apparatus - asymmetric properties of donor and acceptor activities in a cell-free system  Academic Article
  • Chromatin-dependent cooperativity between constitutive and inducible activation domains in CREB  Academic Article
  • Clonal analysis of mechanisms of murine t-helper cell collaboration with effector-cells of macrophage lineage  Academic Article
  • Cyclophilin-A is bound through its peptidylprolyl isomerase domain to the cytoplasmic dynein motor protein complex  Academic Article
  • DNA sequence-directed nucleosome reconstitution on 5S RNA genes of Xenopus-laevis  Academic Article
  • Delayed cutaneous hypersensitivity to cell-free tumor antigens  Academic Article
  • Differential utilization of upstream augs in the beta-secretase mrna suggests that a shunting mechanism regulates translation  Academic Article
  • Direct expression of pcr products in a cell-free transcription/translation system: synthesis of antibacterial peptide cecropin  Academic Article
  • E3 ubiquitin-protein ligase activity of Parkin is dependent on cooperative interaction of RING finger (TRIAD) elements  Academic Article
  • Engineering toward a bacterial "endoplasmic reticulum" for the rapid expression of immunoglobulin proteins  Academic Article
  • Evidence for N-formyl chemotactic peptide-stimulated GTPase activity in human neutrophil homogenates  Academic Article
  • Gene-enzyme relationships of aromatic acid biosynthesis in bacillus-subtilis  Academic Article
  • Genetic and biochemical characterization of mutants of bacillus-subtilis defective in succinate dehydrogenase  Academic Article
  • Glomerular hemodynamics in cell-free and erythrocyte-perfused isolated rat-kidney  Academic Article
  • High-yield Escherichia coli-based cell-free expression of human proteins  Academic Article
  • Homologous recombination in monkey cells and human cell-free-extracts  Academic Article
  • Hyperprotease-producing mutants of bacillus subtilis  Academic Article
  • In vitro synthesis of rous sarcoma virus-specific rna is catalyzed by a DNA-dependent rna polymerase  Academic Article
  • In vitro use of monoclonal-antibodies in Escherichia-coli S-30 extracts to determine the RNA-polymerase sigma-subunit required by a promoter  Academic Article
  • Informatics-based, highly accurate, noninvasive prenatal paternity testing  Academic Article
  • Inhibition of RNA polymerase II transcription in human cells by synthetic DNA-binding ligands  Academic Article
  • Inhibition of nuclear DNA synthesis by an autoantibody to proliferating cell nuclear antigen/cyclin  Academic Article
  • Initiation sites of Rous sarcoma virus RNA-directed DNA synthesis in vitro  Academic Article
  • Interaction of core histones with DNA - equilibrium binding studies  Academic Article
  • Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation  Academic Article
  • Mechanisms underlying responsiveness to tetrahydrobiopterin in mild phenylketonuria mutations  Academic Article
  • Microtubule dynamics and microtubule caps: a time-resolved cryo-electron microscopy study  Academic Article
  • Mitotic repression of transcription in vitro  Academic Article
  • Modulation of human DNA methyltransferase activity and messenger-rna levels in the monoblast cell-line u937 induced to differentiate with dibutyryl-cyclic-amp and phorbol ester  Academic Article
  • Murine cytomegalovirus infection down-regulates mhc class ii expression on macrophages by induction of il-10  Academic Article
  • Mutations in the G-domain of elongation factor G from thermus thermophilus affect both its interaction with GTP and fusidic acid  Academic Article
  • Negative regulation of the Apaf-1 apoptosome by Hsp70  Academic Article
  • Nuclear extracts from globin-synthesizing cells enhance globin transcription in vitro  Academic Article
  • Oligomerization of immunoglobulin-g heavy and light-chains in vitro - a cell-free assay to study the assembly of the endoplasmic-reticulum  Academic Article
  • Opposite facial specificity for 2 hydroquinone epoxidases - (3-si,4-re)-2,5-dihydroxyacetanilide epoxidase from streptomyces ll-c10037 and (3-re,4-si)-2,5-dihydroxyacetanilide epoxidase from streptomyces mpp-3051  Academic Article
  • Physiological studies of biosynthetic indole excretion in bacillus alvei  Academic Article
  • Portable, on-demand biomolecular anufacturing  Academic Article
  • Predicting protein evolution in vitro by phage escape technology  Academic Article
  • Preparation of semiintact chinese hamster ovary cells for reconstitution of endoplasmic reticulum-to-golgi transport in a cell-free system  Academic Article
  • Promoter scanning for transcription inhibition with DNA-binding polyamides  Academic Article
  • Purification and properties of dihydroorotase, a zinc-containing metalloenzyme in clostridium-oroticum  Academic Article
  • Quality-control in the endoplasmic-reticulum - folding and misfolding of vesicular stomatitis-virus g-protein in cells and in vitro  Academic Article
  • RNA tumor virus specific sequences in nuclear DNA of several avian species  Academic Article
  • Reconstitution of the augmin complex provides insights into its architecture and function  Academic Article
  • Reconstitution of the transport of protein between successive compartments of the golgi measured by the coupled incorporation of n-acetylglucosamine  Academic Article
  • Reconstitution of transport of vesicular stomatitis-virus g-protein from the endoplasmic-reticulum to the golgi-complex using a cell-free system  Academic Article
  • Role of lipid trimming and cd1 groove size in cellular antigen presentation  Academic Article
  • Rrna complementarity within mrnas: A possible basis for mrna-ribosome interactions and translational control  Academic Article
  • Rrna-complementarity in the 5 ' untranslated region of mrna specifying the gtx homeodomain protein: Evidence that base-pairing to 18s rrna affects translational efficiency  Academic Article
  • Sequential intermediates in the pathway of intercompartmental transport in a cell-free system  Academic Article
  • Single chemical modifications of the c-1027 enediyne core, a radiomimetic antitumor drug, affect both drug potency and the role of ataxia-telangiectasia mutated in cellular responses to DNA double-strand breaks  Academic Article
  • Single-nucleotide polymorphism-based noninvasive prenatal screening in a high-risk and low-risk cohort  Academic Article
  • Stage-specific assays for coated pit formation and coated vesicle budding in vitro  Academic Article
  • Substrate binding-affinity changes in mitochondrial energy-linked reactions  Academic Article
  • Targeting tumor cell invasion and dissemination in vivo by an aptamer that inhibits urokinase-type plasminogen activator through a novel multifunctional mechanism  Academic Article
  • The cytotoxic cell protease granzyme B initiates apoptosis in a cell-free system by proteolytic processing and activation of the ICE/CED-3 family protease, CPP32, via a novel two-step mechanism  Academic Article
  • The glycoprotein that is transported between successive compartments of the golgi in a cell-free system resides in stacks of cisternae  Academic Article
  • The internal ribosome entry site (ires) contained within the rna-binding motif protein 3 (rbm3) mrna is composed of functionally distinct elements  Academic Article
  • The past, present and future of cell-free protein synthesis  Academic Article
  • Transport-coupled oligosaccharide processing in a cell-free system  Academic Article
  • γ-Secretase modulator (GSM) photoaffinity probes reveal distinct allosteric binding sites on presenilin  Academic Article

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