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Proteomic analysis of plasmodium in the mosquito: progress and pitfalls

Academic Article
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Overview

authors

  • Wass, M. N.
  • Stanway, R.
  • Blagborough, A. M.
  • Lal, K.
  • Prieto, J. H.
  • Raine, D.
  • Sternberg, M. J. E.
  • Talman, A. M.
  • Tomley, F.
  • Yates III, John
  • Sinden, R. E.

publication date

  • August 2012

journal

  • Parasitology  Journal

abstract

  • Here we discuss proteomic analyses of whole cell preparations of the mosquito stages of malaria parasite development (i.e. gametocytes, microgamete, ookinete, oocyst and sporozoite) of Plasmodium berghei. We also include critiques of the proteomes of two cell fractions from the purified ookinete, namely the micronemes and cell surface. Whereas we summarise key biological interpretations of the data, we also try to identify key methodological constraints we have met, only some of which we were able to resolve. Recognising the need to translate the potential of current genome sequencing into functional understanding, we report our efforts to develop more powerful combinations of methods for the in silico prediction of protein function and location. We have applied this analysis to the proteome of the male gamete, a cell whose very simple structural organisation facilitated interpretation of data. Some of the in silico predictions made have now been supported by ongoing protein tagging and genetic knockout studies. We hope this discussion may assist future studies.

subject areas

  • Animals
  • Culicidae
  • Female
  • Insect Vectors
  • Life Cycle Stages
  • Malaria
  • Male
  • Plasmodium berghei
  • Proteomics
  • Protozoan Proteins
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Research

keywords

  • Plasmodium
  • microgamete
  • micronemes
  • mosquito
  • ookinete
  • proteome
  • surface
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Identity

PubMed Central ID

  • PMC3417538

International Standard Serial Number (ISSN)

  • 0031-1820

Digital Object Identifier (DOI)

  • 10.1017/s0031182012000133

PubMed ID

  • 22336136
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Additional Document Info

start page

  • 1131

end page

  • 1145

volume

  • 139

issue

  • 9

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