Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range

Academic Article
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publication date

  • 2012

abstract

  • Single molecule observation of fluorescence resonance energy transfer can be used to provide insight into the structure and dynamics of proteins. Using a straightforward triple-colour labelling strategy, we present a measurement and analysis scheme that can simultaneously study multiple regions within single intrinsically disordered proteins.