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Cyclin D1 associates with the TBP-associated factor TAF(II)250 to regulate Sp1-mediated transcription

Academic Article
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Overview

authors

  • Adnane, J.
  • Shao, Z. H.
  • Robbins, Paul D.

publication date

  • January 1999

journal

  • Oncogene  Journal

abstract

  • We have previously shown that Sp1-mediated transcription is stimulated by Rb and repressed by cyclin D1. The stimulation of Sp1 transcriptional activity by Rb is conferred, in part, through a direct interaction with the TBP-associated factor TAF(II)250. Here we investigated the mechanism(s) through which cyclin D1 represses Sp1. We examined the ability of cyclin D1 to regulate transcription mediated by Gal4-Sp1 fusion proteins, which contain the Gal4 DNA-binding domain and Sp1 trans-activation domain(s). The domain of Sp1 sufficient to confer repression by cyclin D1 was mapped to a region important for interaction with TAF(II)110. We further demonstrate that TAF(II)250-cyclin D1 complexes can be immunoprecipitated from mammalian and baculovirus-infected insect cells and that recombinant GST-TAF(II)250 (amino acids 1-434) associates with cyclin D1 in vitro. Moreover, the overexpression of Rb or CDK4 reduced the level of TAF(II)250-cyclin D1 complex. The amino terminus of cyclin D1 (amino acids 1-100) was sufficient for association with TAF(II)250 and for repressing Sp1-mediated transcription. Taken together, the results suggest that cyclin D1 may regulate transcription by interacting directly or indirectly with TAF(II)250.

subject areas

  • 3T3 Cells
  • Animals
  • Cell Line
  • Cyclin D1
  • Cyclin-Dependent Kinase 4
  • Cyclin-Dependent Kinases
  • DNA-Binding Proteins
  • Gene Expression Regulation
  • Histone Acetyltransferases
  • Humans
  • Mice
  • Mutagenesis
  • Nuclear Proteins
  • Proto-Oncogene Proteins
  • Recombinant Fusion Proteins
  • Retinoblastoma Protein
  • Sp1 Transcription Factor
  • Spodoptera
  • TATA Box
  • TATA-Binding Protein Associated Factors
  • TATA-Box Binding Protein
  • Transcription Factor TFIID
  • Transcription Factors
  • Transcription, Genetic
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Research

keywords

  • Rb
  • Sp1
  • TAF(II)250
  • cyclin D1
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Identity

International Standard Serial Number (ISSN)

  • 0950-9232

Digital Object Identifier (DOI)

  • 10.1038/sj.onc.1202297

PubMed ID

  • 9926939
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Additional Document Info

start page

  • 239

end page

  • 247

volume

  • 18

issue

  • 1

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