Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

CD154 inhibits tumor-induced apoptosis in dendritic cells and tumor growth

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Esche, C.
  • Gambotto, A.
  • Satoh, Y.
  • Gerein, V.
  • Robbins, Paul D.
  • Watkins, S. C.
  • Lotze, M. T.
  • Shurin, M. R.

publication date

  • July 1999

journal

  • European Journal of Immunology  Journal

abstract

  • We have recently demonstrated that murine and human tumors induce apoptosis of dendritic cells (DC). Here, we evaluated the effect of CD40 ligation on the survival of tumor-associated DC and tumor growth. Retroviral transduction of MC38 colon carcinoma cells with the CD154 gene resulted in inhibition of tumor growth. This effect was abrogated in IL-12 knockout mice. Immunohistochemical analysis revealed an increase in CD11c+ (N418) and CD8+ but not NLDC-145+ cells in CD154-transfected tumors in wild-type mice. This increase was less pronounced in IL-12-deficient mice. In vitro, overexpression of CD154 on tumor cells significantly decreased the level of tumor-induced DC apoptosis. Surprisingly, the CD154-induced protection of DC from tumor-induced apoptosis was IL-12 independent in vitro, suggesting an IL-12-dependent and an IL-12-independent mechanism of CD154-induced anti-tumor immunity. Thus, our data suggest a new strategy to improve immunotherapy of cancer by protecting DC from tumor-induced apoptosis.

subject areas

  • Animals
  • Apoptosis
  • CD40 Ligand
  • CD8-Positive T-Lymphocytes
  • Colonic Neoplasms
  • Dendritic Cells
  • Humans
  • Immunotherapy
  • Integrin alphaXbeta2
  • Interleukin-12
  • Male
  • Membrane Glycoproteins
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Transduction, Genetic
scroll to property group menus

Research

keywords

  • CD154
  • IL-12
  • apoptosis
  • dendritic cell
  • immunotherapy
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0014-2980

Digital Object Identifier (DOI)

  • 10.1002/(sici)1521-4141(199907)29:07<2148::aid-immu2148>3.0.co;2-f

PubMed ID

  • 10427977
scroll to property group menus

Additional Document Info

start page

  • 2148

end page

  • 2155

volume

  • 29

issue

  • 7

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support