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Characterization of circulating tumor cell aggregates identified in patients with epithelial tumors

Academic Article
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Overview

authors

  • Cho, E. H.
  • Wendel, M.
  • Luttgen, M.
  • Yoshioka, Craig
  • Marrinucci, Dena
  • Lazar, Daniel
  • Schram, E.
  • Nieva, Jorge Javier
  • Bazhenova, L.
  • Morgan, A.
  • Ko, A. H.
  • Korn, W. M.
  • Kolatkar, A.
  • Bethel, Kelly
  • Kuhn, Peter

publication date

  • February 2012

journal

  • Physical Biology  Journal

abstract

  • Circulating tumor cells (CTCs) have been implicated as a population of cells that may seed metastasis and venous thromboembolism (VTE), two major causes of mortality in cancer patients. Thus far, existing CTC detection technologies have been unable to reproducibly detect CTC aggregates in order to address what contribution CTC aggregates may make to metastasis or VTE. We report here an enrichment-free immunofluorescence detection method that can reproducibly detect and enumerate homotypic CTC aggregates in patient samples. We identified CTC aggregates in 43% of 86 patient samples. The fraction of CTC aggregation was investigated in blood draws from 24 breast, 14 non-small cell lung, 18 pancreatic, 15 prostate stage IV cancer patients and 15 normal blood donors. Both single CTCs and CTC aggregates were measured to determine whether differences exist in the physical characteristics of these two populations. Cells contained in CTC aggregates had less area and length, on average, than single CTCs. Nuclear to cytoplasmic ratios between single CTCs and CTC aggregates were similar. This detection method may assist future studies in determining which population of cells is more physically likely to contribute to metastasis and VTE.

subject areas

  • Adult
  • Cohort Studies
  • Female
  • Fluorescent Antibody Technique
  • Humans
  • Image Interpretation, Computer-Assisted
  • Indoles
  • Keratins
  • Neoplasm Metastasis
  • Neoplasms, Glandular and Epithelial
  • Neoplastic Cells, Circulating
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Identity

PubMed Central ID

  • PMC3387999

International Standard Serial Number (ISSN)

  • 1478-3967

Digital Object Identifier (DOI)

  • 10.1088/1478-3975/9/1/016001

PubMed ID

  • 22306705
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Additional Document Info

start page

  • 016001

volume

  • 9

issue

  • 1

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