related to degree

• Stein, Benjamin, Ph.D. in Chemistry, Scripps Research 2008 - 2015

authors

• Fonslow, B. R.
• Stein, Benjamin
• Webb, K. J.
• Xu, T.
• Choi, J.
• Park, S. K.
• Yates III, John

publication date

• 2013

journal

• Nature Methods  Journal

abstract

• Two major challenges in proteomics are the large number of proteins and their broad dynamic range in the cell. We exploited the abundance-dependent Michaelis-Menten kinetics of trypsin digestion to selectively digest and deplete abundant proteins with a method we call DigDeAPr. We validated the depletion mechanism with known yeast protein abundances, and we observed greater than threefold improvement in low-abundance human-protein identification and quantitation metrics. This methodology should be broadly applicable to many organisms, proteases and proteomic pipelines.

subject areas

• Humans
• Mass Spectrometry
• Peptide Fragments
• Proteins
• Proteome
• Proteomics

PubMed Central ID

• PMC3531578

International Standard Serial Number (ISSN)

• 1548-7091

Digital Object Identifier (DOI)

• 10.1038/nmeth.2250

PubMed ID

• 23160281

start page

• 54

end page

• 56

volume

• 10

issue

• 1