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The retinoblastoma gene-product regulates Sp1-mediated transcription

Academic Article
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Overview

authors

  • Kim, S. J.
  • Onwuta, U. S.
  • Lee, Y. I.
  • Li, R.
  • Botchan, M. R.
  • Robbins, Paul D.

publication date

  • 1992

journal

  • Molecular and Cellular Biology  Journal

abstract

  • We have demonstrated that the retinoblastoma gene product (Rb) can positively regulate transcription from the fourth promoter of the insulinlike growth factor II gene. Two copies of a motif (the retinoblastoma control element) similar to that found in the human c-fos, transforming growth factor beta 1, and c-myc promoters are responsible for conferring Rb regulation to the fourth promoter of the insulinlike growth factor II gene. We have shown that the transcription factor Sp1 can bind to and stimulate transcription from the retinoblastoma control element motif. Moreover, by using a GAL4-Sp1 fusion protein, we have directly demonstrated that Rb positively regulates Sp1 transcriptional activity in vivo. These results indicate that Rb can function as a positive regulator of transcription and that Sp1 is one potential target, either directly or indirectly, for transcriptional regulation by Rb.

subject areas

  • 3T3 Cells
  • Animals
  • Base Sequence
  • Cells, Cultured
  • DNA-Binding Proteins
  • Fungal Proteins
  • Gene Expression Regulation
  • In Vitro Techniques
  • Insulin-Like Growth Factor II
  • Mice
  • Mink
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Promoter Regions, Genetic
  • Regulatory Sequences, Nucleic Acid
  • Retinoblastoma Protein
  • Saccharomyces cerevisiae Proteins
  • Sp1 Transcription Factor
  • Transcription Factors
  • Transcription, Genetic
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Identity

PubMed Central ID

  • PMC364438

International Standard Serial Number (ISSN)

  • 0270-7306

PubMed ID

  • 1588949
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Additional Document Info

start page

  • 2455

end page

  • 2463

volume

  • 12

issue

  • 6

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