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Biosynthesis of asparagine-linked oligosaccharides in saccharomyces-cerevisiae - the alg2 mutation

Academic Article
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Overview

authors

  • Jackson, B. J.
  • Kukuruzinska, M. A.
  • Robbins, Paul D.

publication date

  • August 1993

journal

  • Glycobiology  Journal

abstract

  • In the yeast Saccharomyces cerevisiae, the alg2 mutation causes temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol (Jackson et al., Arch. Biochem. Biophys., 272, 203-209, 1989; Huffaker and Robbins, Proc. Natl. Acad. Sci. USA, 80, 7466-7470, 1983). A gene having the function and genomic location of ALG2 was cloned from libraries based on the multicopy plasmid YEp24 and on the centromere plasmid YCp50. Alg2 mutants transformed with plasmids containing ALG2 regained the capacity to grow and to synthesize lipid-linked oligosaccharides normally at the previously non-permissive temperature. ALG2 was essential for viability in haploid and diploid yeast. The ALG2 gene was transcribed into a single mRNA of 1.7 kb in size. The stability of ALG2 mRNA, assessed after thermal inactivation of RNA polymerase II in an rpb1-1 mutant (Herrick et al., Mol. Cell. Biol., 10, 2269-2284, 1990) was very low, with a t1/2 of < 5 min. The ALG2 transcript accumulation was growth dependent, and it was at least an order of magnitude lower in stationary phase cells compared to exponentially growing cells. The putative translation product of ALG2 contained a potential dolichol recognition domain similar to that found in all three glycosyltransferases of the lipid-linked pathway that have been sequenced.(ABSTRACT TRUNCATED AT 250 WORDS)

subject areas

  • Amino Acid Sequence
  • Asparagine
  • Base Sequence
  • Carbohydrate Sequence
  • Cloning, Molecular
  • Consensus Sequence
  • Dolichol
  • Fungal Proteins
  • Genes, Fungal
  • Genes, Lethal
  • Genes, Regulator
  • Glycosylation
  • Mannosyltransferases
  • Molecular Sequence Data
  • Oligosaccharides
  • Phenotype
  • RNA, Messenger
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Transcription, Genetic
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Research

keywords

  • DOLICHOL
  • LIPID-LINKED OLIGOSACCHARIDES
  • N-GLYCOSYLATION
  • YEAST
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Identity

International Standard Serial Number (ISSN)

  • 0959-6658

Digital Object Identifier (DOI)

  • 10.1093/glycob/3.4.357

PubMed ID

  • 8400550
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Additional Document Info

start page

  • 357

end page

  • 364

volume

  • 3

issue

  • 4

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