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Protein 4.1B expression is induced in mammary epithelial cells during pregnancy and regulates their proliferation

Academic Article
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Overview

authors

  • Kuns, R.
  • Kissil, Joseph
  • Newsham, I. F.
  • Jacks, T.
  • Gutmann, D. H.
  • Sherman, Linda

publication date

  • September 2005

journal

  • Oncogene  Journal

abstract

  • 4.1B is a member of the protein 4.1 superfamily of proteins that link transmembrane proteins to the actin cytoskeleton. The 4.1B gene localizes to chromosome 18p11.3, which undergoes loss of heterozygosity in mammary tumors. Here, we examine the expression of 4.1B in murine mammary epithelium and find that 4.1B is dramatically upregulated in mammary epithelial cells during pregnancy when there is extensive cell proliferation. In contrast, 4.1B is not expressed in virgin, lactating, or involuting mammary epithelium. To examine the consequence of 4.1B loss on mammary epithelial cell proliferation, we analysed mammary glands in 4.1B-null mice. 4.1B loss results in a significant increase in mammary epithelial cell proliferation during pregnancy, but has no effect on mammary epithelial cell proliferation, in virgin or involuting mice. Furthermore, we show that 4.1B inhibits the proliferation of mammary epithelial cell lines by inducing a G1 cell cycle arrest, characterized by decreased cyclin A expression and reduced Rb phosphorylation, and accompanied by reduced erbB2 phosphorylation. This cell cycle arrest does not involve alterations in the activities of MAPK, JNK, or Akt. Collectively, our findings demonstrate that 4.1B regulates mammary epithelial cell proliferation during pregnancy and suggest that its loss may influence mammary carcinoma pathogenesis in multiparous women.

subject areas

  • Animals
  • Breast Neoplasms
  • Cell Proliferation
  • DNA-Binding Proteins
  • Epithelial Cells
  • Female
  • G1 Phase
  • Gene Expression Regulation, Developmental
  • Humans
  • Lactation
  • Mammary Glands, Animal
  • Membrane Proteins
  • Mice
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Microfilament Proteins
  • Phosphorylation
  • Pregnancy
  • Pregnancy, Animal
  • Receptor, ErbB-2
  • Signal Transduction
  • Transcription Factors
  • Tumor Cells, Cultured
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Research

keywords

  • erbB2
  • mammary carcinoma
  • mammary epithelial cells
  • pregnancy
  • protein 4.1B
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Identity

International Standard Serial Number (ISSN)

  • 0950-9232

PubMed ID

  • 16007173
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Additional Document Info

start page

  • 6502

end page

  • 6515

volume

  • 24

issue

  • 43

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