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Improving the comprehensiveness and sensitivity of sheath less capillary electrophoresis-tandem mass spectrometry for proteomic analysis

Academic Article
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Overview

authors

  • Wang, Y.
  • Fonslow, B. R.
  • Wong, C. C. L.
  • Nakorchevsky, A.
  • Yates III, John

publication date

  • October 2012

journal

  • Analytical Chemistry  Journal

abstract

  • We describe a solid phase microextraction (SPME), multistep elution, transient isotachophoresis (tITP) capillary electrophoresis-tandem mass spectrometry (CE-MS/MS) procedure which employs a high sensitivity porous electrospray ionization (ESI) sprayer for the proteomic analysis of a moderately complex protein mixture. In order to improve comprehensiveness and sensitivity over a previously reported proteomic application of the ESI sprayer, we evaluated preconcentration with SPME and multistep elution prior to tITP stacking and CE separation. To maximize separation efficiency, we primarily employed electrokinetic methods for elution and separation after loading the sample by application of pressure. Conditions were developed for optimum simultaneous electrokinetic elution and sample stacking using a tryptic digest of 16 proteins to maximize peptide identifications and minimize band broadening. We performed comparative proteomic analysis of a dilution series using CE and nanoflow liquid chromatography (nLC). We found complementary peptide and protein identifications with larger quantities (100 ng) of a Pyrococcus furiosus tryptic digest, but with mass-limited amounts (5 ng) CE was 3 times more effective at identifying proteins. We attribute these gains in sensitivity to lower noise levels with the porous CE sprayer, illustrated by better signal-to-noise ratios of peptide precursor ions and associated higher XCorr values of identified peptides when compared directly to nLC. From comparative analysis of SPME-tITP-CE with direct injection CE, the SPME-tITP process improved comprehensiveness and sensitivity.

subject areas

  • Animals
  • Archaeal Proteins
  • Electrophoresis, Capillary
  • Escherichia coli
  • Escherichia coli Proteins
  • Isotachophoresis
  • Proteins
  • Proteomics
  • Pyrococcus furiosus
  • Sensitivity and Specificity
  • Solid Phase Microextraction
  • Tandem Mass Spectrometry
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Identity

PubMed Central ID

  • PMC3498465

International Standard Serial Number (ISSN)

  • 0003-2700

Digital Object Identifier (DOI)

  • 10.1021/ac301091m

PubMed ID

  • 23004022
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Additional Document Info

start page

  • 8505

end page

  • 8513

volume

  • 84

issue

  • 20

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