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Apelin, the natural ligand of the orphan seven-transmembrane receptor APJ, inhibits human immunodeficiency virus type 1 entry

Academic Article
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Overview

authors

  • Cayabyab, M.
  • Hinuma, S.
  • Farzan, Michael
  • Choe, Hyeryun
  • Fukusumi, S.
  • Kitada, C.
  • Nishizawa, N.
  • Hosoya, M.
  • Nishimura, O.
  • Messele, T.
  • Pollakis, G.
  • Goudsmit, J.
  • Fujino, M.
  • Sodroski, J.

publication date

  • December 2000

journal

  • Journal of Virology  Journal

abstract

  • In addition to the CCR5 and CXCR4 chemokine receptors, a subset of primary human immunodeficiency virus type 1 (HIV-1) isolates can also use the seven-transmembrane-domain receptor APJ as a coreceptor. A previously identified ligand of APJ, apelin, specifically inhibited the entry of primary T-tropic and dualtropic HIV-1 isolates from different clades into cells expressing CD4 and APJ. Analysis of apelin analogues demonstrated that potent and specific antiviral activity was retained by a 13-residue, arginine-rich peptide. Antiviral potency was influenced by the integrity of methionine 75, which contributes to APJ-binding affinity, and by the retention of apelin residues 63 to 65. These studies demonstrate the ability of a small peptide ligand to block the function of APJ as an HIV-1 coreceptor, identify apelin sequences important for the inhibition, and provide new reagents for the investigation of the significance of APJ to HIV-1 infection and pathogenesis.

subject areas

  • Acquired Immunodeficiency Syndrome
  • Amino Acid Sequence
  • Carrier Proteins
  • HIV-1
  • Humans
  • Intercellular Signaling Peptides and Proteins
  • Ligands
  • Molecular Sequence Data
  • Receptors, Dopamine D2
  • Receptors, G-Protein-Coupled
  • Receptors, Virus
  • Virus Replication
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Identity

PubMed Central ID

  • PMC112482

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/jvi.74.24.11972-11976.2000

PubMed ID

  • 11090199
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Additional Document Info

start page

  • 11972

end page

  • 11976

volume

  • 74

issue

  • 24

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