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Hemagglutinin homologue from h17n10 bat influenza virus exhibits divergent receptor-binding and ph-dependent fusion activities

Academic Article
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Overview

authors

  • Zhu, X. Y.
  • Yu, W. L.
  • McBride, R.
  • Li, Yuxing
  • Chen, L. M.
  • Donis, R. O.
  • Tong, S. X.
  • Paulson, James
  • Wilson, Ian

publication date

  • January 2013

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • Bat influenza virus H17N10 represents a distinct lineage of influenza A viruses with gene segments coding for proteins that are homologs of the surface antigens, hemagglutinin (HA) and neuraminidase (NA). Our recent study of the N10 NA homolog revealed an NA-like structure, but with a highly divergent putative active site exhibiting little or no NA activity, and provided strong motivation for performing equivalent structural and functional analyses of the H17 HA protein. The overall structure of the H17 HA homolog from A/little yellow-shouldered bat/Guatemala/060/2010 at 3.18 Å resolution is very similar to other influenza HAs, with a putative receptor-binding site containing some conserved aromatic residues that form the base of the sialic acid binding site. However, the rest of the H17 receptor-binding site differs substantially from the other HA subtypes, including substitution of other conserved residues associated with receptor binding. Significantly, electrostatic potential analyses reveal that this putative receptor-binding site is highly acidic, making it unfavorable to bind any negatively charged sialylated receptors, consistent with the recombinant H17 protein exhibiting no detectable binding to sialylated glycans. Furthermore, the fusion mechanism is also distinct; trypsin digestion with recombinant H17 protein, when exposed to pH 4.0, did not degrade the HA1 and HA2, in contrast to other HAs. These distinct structural features and functional differences suggest that the H17 HA behaves very differently compared with other influenza HAs.

subject areas

  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Chiroptera
  • Crystallography, X-Ray
  • Hemagglutinin Glycoproteins, Influenza Virus
  • Hydrogen-Ion Concentration
  • Influenza A virus
  • Membrane Fusion
  • Models, Molecular
  • Molecular Sequence Data
  • Protein Structure, Quaternary
  • Receptors, Virus
  • Sequence Homology, Amino Acid
  • Sialic Acids
  • Static Electricity
  • Viral Fusion Proteins
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Research

keywords

  • crystal structure
  • evolution
  • infection
  • protease susceptibility
  • viral entry
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Identity

PubMed Central ID

  • PMC3557073

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.1218509110

PubMed ID

  • 23297216
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Additional Document Info

start page

  • 1458

end page

  • 1463

volume

  • 110

issue

  • 4

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