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Synthesis of bispecific antibodies using genetically encoded unnatural amino acids

Academic Article
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Overview

related to degree

  • Wold, Erik, Ph.D. in Chemistry, Scripps Research 2009 - 2015
  • Axup, Jun Yin, Ph.D. in Chemical Biology, Scripps Research 2008 - 2013
  • Kazane, Stephanie Ann, Ph.D. in Chemical Biology, Scripps Research 2007 - 2012

authors

  • Kim, C. H.
  • Axup, Jun Yin
  • Dubrovska, A.
  • Kazane, Stephanie Ann
  • Hutchins, B. A.
  • Wold, Erik
  • Smider, Vaughn
  • Schultz, Peter

publication date

  • June 2012

journal

  • Journal of the American Chemical Society  Journal

abstract

  • Bispecific antibodies were constructed using genetically encoded unnatural amino acids with orthogonal chemical reactivity. A two-step process afforded homogeneous products in excellent yield. Using this approach, we synthesized an anti-HER2/anti-CD3 bispecific antibody, which efficiently cross-linked HER2+ cells and CD3+ cells. In vitro effector-cell mediated cytotoxicity was observed at picomolar concentrations.

subject areas

  • Amino Acids
  • Antibodies, Bispecific
  • Antigens, CD3
  • Cell Line
  • Cell Line, Tumor
  • Cytotoxicity, Immunologic
  • Humans
  • Models, Molecular
  • Receptor, ErbB-2
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Identity

PubMed Central ID

  • PMC4299457

International Standard Serial Number (ISSN)

  • 0002-7863

Digital Object Identifier (DOI)

  • 10.1021/ja303904e

PubMed ID

  • 22642368
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Additional Document Info

start page

  • 9918

end page

  • 9921

volume

  • 134

issue

  • 24

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