Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Bacterial expression of tenecin 3, an insect antifungal protein isolated from tenebrio molitor, and its efficient purification

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Kim, Dae Hee
  • Lee, Y. T.
  • Lee, Y. J.
  • Chung, J. H.
  • Lee, B. L.
  • Choi, B. S.
  • Lee, Y.

publication date

  • December 1998

journal

  • Molecules and Cells  Journal

abstract

  • Tenecin 3, an antifungal protein isolated from the insect Tenebrio molitor larvae, inhibits growth of the fungus Candida albicans. However, the antifungal mechanism and functions of tenecin 3 have not yet been studied due to its very low availability from the natural source. Here we report an expression system of the recombinant tenecin 3 in E. coli, whose amino acid composition is the same with that of the natural tenecin 3. We also devised a simple and easy procedure to isolate the recombinant protein from the bacterial cell extracts. The recombinant tenecin 3 showed an antifungal activity against C. albicans as the natural tenecin 3 did. Therefore large quantities of tenecin 3 can be easily obtained by the expression and purification system of tenecin 3 described in this report.

subject areas

  • Amino Acid Sequence
  • Animals
  • Antifungal Agents
  • Base Sequence
  • Candida albicans
  • Cell Division
  • Cloning, Molecular
  • DNA, Complementary
  • Dose-Response Relationship, Drug
  • Escherichia coli
  • Gene Expression Regulation, Bacterial
  • Insect Proteins
  • Plasmids
  • Recombinant Fusion Proteins
  • Tenebrio
scroll to property group menus

Research

keywords

  • Escherichia coli
  • Tenebrio molitor
  • insect antifungal protein
  • recombinant protein
  • tenecin 3
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 1016-8478

PubMed ID

  • 9895135
scroll to property group menus

Additional Document Info

start page

  • 786

end page

  • 789

volume

  • 8

issue

  • 6

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support