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Chronic ethanol enhances muscarinic receptor-mediated activator protein-1 (ap-1) DNA binding in cerebellar granule cells

Academic Article
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Overview

authors

  • Cebers, G.
  • Hou, N. Y.
  • Cebere, A.
  • Terenius, Lars
  • Liljequist, S.

publication date

  • 1999

journal

  • European Journal of Pharmacology  Journal

abstract

  • In this study we investigated effects of acute and chronic ethanol exposure on carbachol-induced activator protein-1 (AP-1) DNA binding in rat cerebellar granule cells. Acute ethanol application did not alter, whereas chronic ethanol exposure potentiated the carbachol-induced AP-1 DNA binding. The protein composition of the AP-1 transcription factor complex activated by carbachol stimulation of muscarinic receptors was analysed in control and chronic ethanol-exposed cells using a supershift assay with specific antibodies against c-Fos, Fos B, c-Jun, Jun B and Jun D proteins. Supershift analysis revealed that the carbachol-induced AP-1 complex was composed predominantly of Jun D and c-Fos. The composition of the AP-1 complex activated by carbachol in chronic ethanol-exposed cells did not differ from control. These findings indicate that chronic ethanol treatment can modulate carbachol-induced AP-1 DNA binding activity in cerebellar granule cells.

subject areas

  • Animals
  • Cells, Cultured
  • Central Nervous System Depressants
  • Cerebellum
  • DNA
  • Ethanol
  • Immediate-Early Proteins
  • Oligonucleotides
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Muscarinic
  • Transcription Factor AP-1
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Research

keywords

  • (rat)
  • AP-1 (activator protein-1)
  • carbachol
  • cerebellar granule cell
  • ethanol
  • muscarinic receptor
  • transcription factor
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Identity

International Standard Serial Number (ISSN)

  • 0014-2999

Digital Object Identifier (DOI)

  • 10.1016/s0014-2999(99)00632-9

PubMed ID

  • 10585535
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Additional Document Info

start page

  • 203

end page

  • 208

volume

  • 383

issue

  • 2

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