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Repression of the IgH enhancer in teratocarcinoma cells associated with a novel octamer factor

Academic Article
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Overview

authors

  • Lenardo, M. J.
  • Staudt, L.
  • Robbins, Paul D.
  • Kuang, A.
  • Mulligan, R. C.
  • Baltimore, D.

publication date

  • January 1989

journal

  • Science  Journal

abstract

  • Embryonal carcinoma (EC) cell lines are models for early cells in mouse embryogenesis. A 300-base pair fragment of the heavy chain enhancer was inactive in F9 EC cells, unlike in other nonlymphoid cells where it has significant activity. Alterations of the octamer motif increased enhancer activity. Nuclear extracts from F9 cells contained an octamer binding protein (NF-A3) that was unique to EC cells; the amount of NF-A3 decreased upon differentiation. It is proposed that NF-A3 represses specific regulatory sequences that contain the octamer motif. Thus, the same DNA sequence mediates either negative or positive transcriptional effects, depending on the cell type.

subject areas

  • Animals
  • Bucladesine
  • Cell Differentiation
  • DNA
  • Embryonal Carcinoma Stem Cells
  • Enhancer Elements, Genetic
  • Immunoglobulin Heavy Chains
  • Macromolecular Substances
  • Mice
  • Mutation
  • Neoplastic Stem Cells
  • RNA, Messenger
  • Regulatory Sequences, Nucleic Acid
  • Repressor Proteins
  • Transcription, Genetic
  • Transfection
  • Tretinoin
  • Tumor Cells, Cultured
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Identity

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.2536195

PubMed ID

  • 2536195
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Additional Document Info

start page

  • 544

end page

  • 546

volume

  • 243

issue

  • 4890

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