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Muscle-based gene therapy and tissue engineering for treatment of growth plate injuries

Academic Article
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Overview

authors

  • Lee, C. W.
  • Martinek, V.
  • Usas, A.
  • Musgrave, D.
  • Pickvance, E. A.
  • Robbins, Paul D.
  • Moreland, M. S.
  • Fu, F. H.
  • Huard, J.

publication date

  • September 2002

journal

  • Journal of Pediatric Orthopaedics  Journal

abstract

  • Growth plate injuries may lead to a progressive angular deformity or longitudinal growth disturbance. The authors investigated the feasibility of gene therapy and tissue engineering based on autologous muscle-and adenoviral-mediated gene transfer of insulin-like growth factor-1 (IGF-1) and bone morphogenetic protein-2 (BMP-2) to treat tibial physeal defects in rabbits. The medial half of the left proximal tibial growth plate was completely excised in 44 6-week-old New Zealand white rabbits. Four experimental groups were created: no treatment (I), autologous muscle interposition (II), autologous muscle interposition injected with adIGF-1 (III), and autologous muscle interposition injected with adBMP-2 (IV). Radiographic and histologic assessments were obtained postoperatively. Significant tibial shortening and a compact osseous bridge were observed in groups I and IV. Growth plates remained open in groups II and III. This experiment demonstrates that IGF-1 had a supportive effect on physeal chondrocytes, while BMP-2 caused increased osteogenic activity in the injured growth plates.

subject areas

  • Adenoviridae
  • Animals
  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Proteins
  • Feasibility Studies
  • Female
  • Gene Transfer Techniques
  • Growth Plate
  • Insulin-Like Growth Factor I
  • Rabbits
  • Tibia
  • Tissue Engineering
  • Transforming Growth Factor beta
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Research

keywords

  • BMP-2
  • IGF-1
  • gene therapy
  • muscle
  • physeal defect
  • tissue engineering
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Identity

International Standard Serial Number (ISSN)

  • 0271-6798

Digital Object Identifier (DOI)

  • 10.1097/01.bpo.0000026337.10173.bd

PubMed ID

  • 12198455
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Additional Document Info

start page

  • 565

end page

  • 572

volume

  • 22

issue

  • 5

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