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Decoding human cytomegalovirus

Academic Article
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Overview

authors

  • Stern-Ginossar, N.
  • Weisburd, B.
  • Michalski, A.
  • Vu, T. K. L.
  • Hein, M. Y.
  • Huang, S. X.
  • Ma, M.
  • Shen, Ben
  • Qian, S. B.
  • Hengel, H.
  • Mann, M.
  • Ingolia, N. T.
  • Weissman, J. S.

publication date

  • November 2012

journal

  • Science  Journal

abstract

  • The human cytomegalovirus (HCMV) genome was sequenced 20 years ago. However, like those of other complex viruses, our understanding of its protein coding potential is far from complete. We used ribosome profiling and transcript analysis to experimentally define the HCMV translation products and follow their temporal expression. We identified hundreds of previously unidentified open reading frames and confirmed a fraction by means of mass spectrometry. We found that regulated use of alternative transcript start sites plays a broad role in enabling tight temporal control of HCMV protein expression and allowing multiple distinct polypeptides to be generated from a single genomic locus. Our results reveal an unanticipated complexity to the HCMV coding capacity and illustrate the role of regulated changes in transcript start sites in generating this complexity.

subject areas

  • Alternative Splicing
  • Cytomegalovirus
  • Cytomegalovirus Infections
  • Genetic Variation
  • Genome, Viral
  • Humans
  • Open Reading Frames
  • Protein Biosynthesis
  • Proteome
  • Sequence Analysis, DNA
  • Transcription, Genetic
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Identity

PubMed Central ID

  • PMC3817102

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.1227919

PubMed ID

  • 23180859
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Additional Document Info

start page

  • 1088

end page

  • 1093

volume

  • 338

issue

  • 6110

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