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MicroRNA-221-222 regulate the cell cycle in mast cells

Academic Article
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Overview

authors

  • Mayoral, R. J.
  • Pipkin, Matthew
  • Pachkov, M.
  • van Nimwegen, E.
  • Rao, A.
  • Monticelli, S.

publication date

  • January 2009

journal

  • Journal of Immunology  Journal

abstract

  • MicroRNAs (miRNAs) constitute a large family of small noncoding RNAs that have emerged as key posttranscriptional regulators in a wide variety of organisms. Because any one miRNA can potentially regulate expression of a distinct set of genes, differential miRNA expression can shape the repertoire of proteins that are actually expressed during development and differentiation or disease. Here, we have used mast cells as a model to investigate the role of miRNAs in differentiated innate immune cells and found that miR-221-222 are significantly up-regulated upon mast cell activation. Using both bioinformatics and experimental approaches, we identified some signaling pathways, transcription factors, and potential cis-regulatory regions that control miR-221-222 transcription. Overexpression of miR-221-222 in a model mast cell line perturbed cell morphology and cell cycle regulation without altering viability. While in stimulated cells miR-221-222 partially counteracted expression of the cell-cycle inhibitor p27(kip1), we found that in the mouse alternative splicing results in two p27(kip1) mRNA isoforms that differ in their 3' untranslated region, only one of which is subject to miR-221-222 regulation. Additionally, transgenic expression of miR-221-222 from bacterial artificial chromosome clones in embryonic stem cells dramatically reduced cell proliferation and severely impaired their accumulation. Our study provides further insights on miR-221-222 transcriptional regulation as well as evidences that miR-221-222 regulate cell cycle checkpoints in mast cells in response to acute activation stimuli.

subject areas

  • Animals
  • Bone Marrow Cells
  • Cell Aggregation
  • Cell Cycle
  • Cell Line
  • Cell Proliferation
  • Cell Survival
  • Gene Expression Regulation
  • Growth Inhibitors
  • Humans
  • Lymphocyte Subsets
  • Mast Cells
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • MicroRNAs
  • Signal Transduction
  • Transcriptional Activation
  • Up-Regulation
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Identity

PubMed Central ID

  • PMC2610349

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 19109175
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Additional Document Info

start page

  • 433

end page

  • 445

volume

  • 182

issue

  • 1

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