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KlenTaq polymerase replicates unnatural base pairs by inducing a Watson-Crick geometry

Academic Article
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Overview

related to degree

  • Malyshev, Denis A, Ph.D. in Chemical Biology, Scripps Research 2008 - 2013

authors

  • Betz, K.
  • Malyshev, Denis A
  • Lavergne, T.
  • Welte, W.
  • Diederichs, K.
  • Dwyer, T. J.
  • Ordoukhanian, Phillip
  • Romesberg, Floyd
  • Marx, A.

publication date

  • July 2012

journal

  • Nature Chemical Biology  Journal

abstract

  • Many candidate unnatural DNA base pairs have been developed, but some of the best-replicated pairs adopt intercalated structures in free DNA that are difficult to reconcile with known mechanisms of polymerase recognition. Here we present crystal structures of KlenTaq DNA polymerase at different stages of replication for one such pair, dNaM-d5SICS, and show that efficient replication results from the polymerase itself, inducing the required natural-like structure.

subject areas

  • Base Pairing
  • DNA
  • Models, Molecular
  • Nucleic Acid Conformation
  • Taq Polymerase
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Identity

PubMed Central ID

  • PMC3690913

International Standard Serial Number (ISSN)

  • 1552-4450

Digital Object Identifier (DOI)

  • 10.1038/nchembio.966

PubMed ID

  • 22660438
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Additional Document Info

start page

  • 612

end page

  • 614

volume

  • 8

issue

  • 7

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