Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Retrovirus-mediated gene transfer in lungs of living fetal sheep

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Pitt, B. R.
  • Schwarz, M. A.
  • Pilewski, J. M.
  • Nakayama, D.
  • Mueller, G. M.
  • Robbins, Paul D.
  • Watkins, S. A.
  • Albertine, K. H.
  • Bland, R. D.

publication date

  • July 1995

journal

  • Gene Therapy  Journal

abstract

  • In utero somatic gene transfer may be a useful therapeutic strategy for a variety of inherited disorders. In the present study, we demonstrate transgene expression in the airways of fetal lamb lungs, 2-3 weeks after injection of Moloney murine leukemia retrovirus based vectors containing cDNA for beta-galactosidase (lacZ) or human interleukin receptor antagonist protein (IRAP), into the fluid filled future airspace of fully catheterized twin fetal lambs (104-117 days gestational age; term 147 days). Expression of lacZ or IRAP was limited to the twin that received the respective vector and was apparent, at light microscopic level, in the epithelium and submucosal space of proximal airways, and to a lesser extent, in the respiratory epithelium of the distal airways. These data demonstrate for the first time that transfer of foreign DNA to fetal lung can be accomplished. These findings support the use of retroviral vectors for somatic lung DNA transfer and suggest that inherited disorders such as cystic fibrosis may be approached therapeutically via gene transfer, in utero.

subject areas

  • Animals
  • Cell Division
  • Defective Viruses
  • Fetus
  • Gene Expression
  • Gene Transfer Techniques
  • Genetic Therapy
  • Genetic Vectors
  • Humans
  • Immunohistochemistry
  • Interleukin 1 Receptor Antagonist Protein
  • Lac Operon
  • Lung
  • Respiratory Tract Diseases
  • Retroviridae
  • Sheep
  • Sialoglycoproteins
  • beta-Galactosidase
scroll to property group menus

Research

keywords

  • EPITHELIUM
  • FETAL
  • LUNG
  • RETROVIRUS
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0969-7128

PubMed ID

  • 7671110
scroll to property group menus

Additional Document Info

start page

  • 344

end page

  • 350

volume

  • 2

issue

  • 5

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support