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Mining human antibody repertoires

Academic Article
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Overview

authors

  • Beerli, R. R.
  • Rader, Christoph

publication date

  • July 2010

journal

  • MAbs  Journal

abstract

  • Human monoclonal antibodies (mAbs) have become drugs of choice for the management of an increasing number of human diseases.  Human antibody repertoires provide a rich source for human mAbs.  Here we review the characteristics of natural and non-natural human antibody repertoires and their mining with non-combinatorial and combinatorial strategies.  In particular, we discuss the selection of human mAbs from naïve, immune, transgenic, and synthetic human antibody repertoires using methods based on hybridoma technology, clonal expansion of peripheral B cells, single-cell PCR, phage display, yeast display, and mammalian cell display.  Our reliance on different strategies is shifting as we gain experience and refine methods to the efficient generation of human mAbs with superior pharmacokinetic and pharmacodynamic properties.

subject areas

  • Animals
  • Antibodies, Monoclonal
  • B-Lymphocytes
  • Clonal Selection, Antigen-Mediated
  • Combinatorial Chemistry Techniques
  • Humans
  • Hybridomas
  • Immunotherapy
  • Peptide Library
  • Yeasts
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Research

keywords

  • B cells
  • antibody engineering
  • antibody libraries
  • display technologies
  • human monoclonal antibodies
  • hybridoma technology
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Identity

PubMed Central ID

  • PMC3180084

International Standard Serial Number (ISSN)

  • 1942-0862

Digital Object Identifier (DOI)

  • 10.4161/mabs.2.4.12187

PubMed ID

  • 20505349
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Additional Document Info

start page

  • 365

end page

  • 378

volume

  • 2

issue

  • 4

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