Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Direct repeat sequences are implicated in the regulation of 2 streptomyces chitinase promoters that are subject to carbon catabolite control

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Delic, I.
  • Robbins, Paul D.
  • Westpheling, J.

publication date

  • March 1992

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • We report the identification and partial characterization of the promoters for two chitinase genes from Streptomyces plicatus. Chitinases are a family of enzymes made by Streptomyces and other soil microbes to digest chitin, an abundant source of carbon and nitrogen in the soil. The promoter regions of two chitinases were defined by using transcriptional fusions to the xylE reporter gene. Transcription was shown to be glucose-sensitive and chitin-dependent. Each promoter contains a putative RNA polymerase binding site with a recognition sequence very similar to that observed in many eubacterial vegetatively expressed genes. In both promoters, a pair of 12-base-pair direct repeat sequences overlap the putative RNA polymerase binding sites. Further analysis of one of the promoters revealed that a single-base change within the direct repeat sequences resulted in glucose-resistant, chitin-independent expression in vivo. In addition, the promoter region that includes the direct repeat sequences was shown to interact with a sequence-specific DNA binding factor in vitro. Similar direct repeat sequences are present in other chitinase genes recently characterized, and we suggest that these repeats may be involved in repression and induction for this entire class of catabolite-controlled genes.

subject areas

  • Base Sequence
  • Carbon
  • Chitinase
  • DNA, Bacterial
  • DNA-Binding Proteins
  • Gene Expression Regulation, Bacterial
  • Glucose
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Oligodeoxyribonucleotides
  • Promoter Regions, Genetic
  • Repetitive Sequences, Nucleic Acid
  • Streptomyces
  • Transcription, Genetic
scroll to property group menus

Research

keywords

  • CARBOHYDRATE DEGRADATION
  • PRIMARY METABOLISM
  • TRANSCRIPTIONAL REGULATION
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.89.5.1885

PubMed ID

  • 1542688
scroll to property group menus

Additional Document Info

start page

  • 1885

end page

  • 1889

volume

  • 89

issue

  • 5

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support