Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Prodynorphin storage and processing in axon terminals and dendrites

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Yakovleva, T.
  • Bazov, I.
  • Cebers, G.
  • Marinova, Z.
  • Hara, Y.
  • Ahmed, A.
  • Vlaskovska, M.
  • Johansson, B. R.
  • Hochgeschwender, U.
  • Singh, I. N.
  • Bruce-Keller, A. J.
  • Hurd, Y. L.
  • Kaneko, T.
  • Terenius, Lars
  • Ekstrom, T. J.
  • Hauser, K. F.
  • Pickel, V. M.
  • Bakalkin, G.

publication date

  • October 2006

journal

  • FASEB Journal  Journal

abstract

  • The classical view postulates that neuropeptide precursors in neurons are processed into mature neuropeptides in the somatic trans-Golgi network (TGN) and in secretory vesicles during axonal transport. Here we show that prodynorphin (PDYN), precursor to dynorphin opioid peptides, is predominantly located in axon terminals and dendrites in hippocampal and striatal neurons. The molar content of unprocessed PDYN was much greater than that of dynorphin peptides in axon terminals of PDYN-containing neurons projecting to the CA3 region of the hippocampus and in the striatal projections to the ventral tegmental area. Electron microscopy showed coexistence of PDYN and dynorphins in the same axon terminals with occasional codistribution in individual dense core vesicles. Thus, the precursor protein is apparently stored at presynaptic sites. In comparison with the hippocampus and striatum, PDYN and dynorphins were more equally distributed between neuronal somata and processes in the amygdala and cerebral cortex, suggesting regional differences in the regulation of trafficking and processing of the precursor protein. Potassium-induced depolarization activated PDYN processing and secretion of opioid peptides in neuronal cultures and in a model cell line. Regulation of PDYN storage and processing at synapses by neuronal activity or extracellular stimuli may provide a local mechanism for regulation of synaptic transmission.

subject areas

  • Animals
  • Basal Ganglia
  • Brain
  • Cells, Cultured
  • Dendrites
  • Enkephalins
  • Hippocampus
  • Male
  • Membrane Potentials
  • Microscopy, Electron
  • Neurons
  • Presynaptic Terminals
  • Protein Precursors
  • Rats
  • Rats, Sprague-Dawley
  • Tissue Distribution
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0892-6638

Digital Object Identifier (DOI)

  • 10.1096/fj.06-6174fje

PubMed ID

  • 16966485
scroll to property group menus

Additional Document Info

start page

  • 2124

end page

  • 6

volume

  • 20

issue

  • 12

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support