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Nuclear membrane proteins with potential disease links found by subtractive proteomics

Academic Article
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Overview

authors

  • Schirmer, E. C.
  • Florens, L.
  • Guan, T. L.
  • Yates III, John
  • Gerace, Larry

publication date

  • September 2003

journal

  • Science  Journal

abstract

  • To comprehensively identify integral membrane proteins of the nuclear envelope (NE), we prepared separately NEs and organelles known to cofractionate with them from liver. Proteins detected by multidimensional protein identification technology in the cofractionating organelles were subtracted from the NE data set. In addition to all 13 known NE integral proteins, 67 uncharacterized open reading frames with predicted membrane-spanning regions were identified. All of the eight proteins tested targeted to the NE, indicating that there are substantially more integral proteins of the NE than previously thought. Furthermore, 23 of these mapped within chromosome regions linked to a variety of dystrophies.

subject areas

  • Algorithms
  • Animals
  • Cell Fractionation
  • Chromosome Mapping
  • DNA, Complementary
  • Genetic Diseases, Inborn
  • Genetic Linkage
  • Humans
  • Liver
  • Membrane Proteins
  • Mice
  • Mice, Inbred Strains
  • Nuclear Envelope
  • Nuclear Proteins
  • Open Reading Frames
  • Protein Structure, Tertiary
  • Proteomics
  • Rats
  • Rats, Sprague-Dawley
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Identity

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.1088176

PubMed ID

  • 12958361
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Additional Document Info

start page

  • 1380

end page

  • 1382

volume

  • 301

issue

  • 5638

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