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Platelet-activating factor induces NF-kappa B activation through a G protein-coupled pathway

Academic Article
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Overview

authors

  • Kravchenko, Vladimir
  • Pan, Z. X.
  • Han, Jiahuai
  • Herbert, J. M.
  • Ulevitch, Richard
  • Ye, R. D.

publication date

  • June 1995

journal

  • Journal of Biological Chemistry  Journal

abstract

  • The capability of platelet-activating factor (PAF) to induce transcription factor activation was examined. In stably transfected Chinese hamster ovary cells expressing the PAF receptor (CHO-PAFR), PAF stimulation resulted in the nuclear expression of a DNA binding activity with specificity to the kappa B sequence. The p50 and p65 proteins, constituents of the prototypic nuclear factor kappa B (NF-kappa B), were identified as components of the DNA protein complexes by antipeptide antibodies in gel supershift as well as UV cross-linking experiments. PAF induced an initial decrease and subsequent increase of cytoplasmic I kappa B alpha levels, accompanied by up-regulation of the I kappa B alpha messenger RNA, a feature of NF-kappa B activation. PAF-induced kappa B binding activity was detected within 15 min after agonist stimulation, peaked at 30-40 min, and remained detectable by 2.5 h. SR 27417, a PAF receptor antagonist, blocked PAF-induced kappa B binding activity but not that induced by tumor necrosis factor-alpha (TNF alpha). Cholera toxin treatment markedly reduced PAF-induced kappa B binding activity, whereas pertussis toxin had no significant inhibitory effect. Neither of the two toxins affected the kappa B binding activity induced by TNF alpha in the same cells. In addition to the CHO-PAFR cells, PAF stimulated kappa B binding activity in the murine P388D1 macrophage and the human ASK.0 B cell lines that express endogenous PAF receptors. These results imply a potential role of PAF in the regulation of gene expression through a G protein-coupled transcription factor activation pathway.

subject areas

  • Animals
  • Base Sequence
  • Binding Sites
  • CHO Cells
  • Cell Line
  • Cell Nucleus
  • Cricetinae
  • DNA
  • GTP-Binding Proteins
  • Gene Expression
  • Humans
  • Kinetics
  • Macrophages
  • Mice
  • Molecular Sequence Data
  • NF-kappa B
  • Oligodeoxyribonucleotides
  • Platelet Activating Factor
  • Platelet Membrane Glycoproteins
  • Promoter Regions, Genetic
  • Receptors, Cell Surface
  • Receptors, G-Protein-Coupled
  • Recombinant Proteins
  • Transfection
  • Tumor Necrosis Factor-alpha
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Identity

International Standard Serial Number (ISSN)

  • 0021-9258

PubMed ID

  • 7797472
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Additional Document Info

start page

  • 14928

end page

  • 14934

volume

  • 270

issue

  • 25

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