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Influence of three-dimensional structure on the immunogenicity of a peptide expressed on the surface of a plant virus

Academic Article
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Overview

related to degree

  • Giesing Chokeir, Heidi, Ph.D. in Macromolecular and Cellular Structure and Chemistry, Scripps Research 1995 - 2002

authors

  • Taylor, K. M.
  • Lin, T. W.
  • Porta, C.
  • Mosser, A. G.
  • Giesing Chokeir, Heidi
  • Lomonossoff, G. P.
  • Johnson Jr., John

publication date

  • March 2000

journal

  • Journal of Molecular Recognition  Journal

abstract

  • The influence of peptide structure on immunogenicity has been investigated by constructing a series of cowpea mosaic virus (CPMV) chimaeras expressing the 14 amino acid NIm-1A epitope from human rhinovirus 14 (HRV-14) at different positions on the capsid surface. Biochemical and crystallographic analysis of a CPMV/HRV chimaera expressing the NIm-1A epitope inserted into the betaC'-betaC" loop of the S protein revealed that, although the inserted peptide was free at its C-terminus, it adopted a conformation distinct from that previously found when a similarly cleaved peptide was expressed in the betaB-betaC loop of the S protein. Adjustment of the site of insertion within the betaB-betaC loop resulted in the isolation of a chimaera in which cleavage at the C-terminus of the epitope was much reduced. Crystallographic analysis confirmed that in this case the epitope was presented as a closed loop. Polyclonal antisera raised against the CPMV/ HRV chimaera presenting the NIm-1A epitope as a closed loop had a significantly enhanced ability to bind to intact HRV-14 particles compared with antisera raised against chimaeras presenting the same sequence as peptides with free C-termini. These results demonstrate that the mode of presentation of an epitope on a heterologous carrier can dramatically affect its immunological properties.

subject areas

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Capsid
  • Comovirus
  • Crystallography, X-Ray
  • Electrophoresis, Polyacrylamide Gel
  • Epitopes
  • Humans
  • Immune Sera
  • Injections, Intramuscular
  • Molecular Sequence Data
  • Peptides
  • Protein Conformation
  • Rabbits
  • Recombinant Fusion Proteins
  • Rhinovirus
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Research

keywords

  • X-ray crystallography
  • antibody binding
  • cowpea mosaic virus
  • epitope presentation
  • human rhinovirus 14 NIm-1A epitope
  • immunogenicity
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Identity

International Standard Serial Number (ISSN)

  • 0952-3499

Digital Object Identifier (DOI)

  • 10.1002/(sici)1099-1352(200003/04)13:2<71::aid-jmr489>3.3.co;2-m

PubMed ID

  • 10822251
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Additional Document Info

start page

  • 71

end page

  • 82

volume

  • 13

issue

  • 2

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