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Isotypes of a-tubulin are differentially regulated during neuronal maturation

Academic Article
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Overview

authors

  • Bloom, Floyd
  • Durand, M.
  • Miller, F. D.
  • Milner, R, J.
  • Naus, C. C. G.

publication date

  • December 1987

journal

  • Journal of Cell Biology  Journal

abstract

  • The mRNAs for two isotypes of alpha-tubulin, termed T alpha 1 and T26, are known to be expressed in the rat nervous system. We have compared the expression of these two alpha-tubulin mRNAs during neural development, using RNA blotting and in situ hybridization techniques with probes directed against unique sequences of each mRNA. T alpha 1 mRNA is highly enriched in the embryonic nervous system but is markedly less abundant in the adult brain; T26 mRNA is expressed in many embryonic tissues with little change in abundance during development. Within the nervous system, T alpha 1 mRNA is enriched in regions with neurons actively undergoing neurite extension, such as the cortical plate, whereas T26 mRNA is relatively homogeneous in distribution, with some enrichment in proliferative zones. Expression of T alpha 1 mRNA is also increased in PC12 cells induced to differentiate and extend neurite processes by nerve growth factor. Taken together, the data indicate that T alpha 1-tubulin mRNA is expressed at high levels during the extension of neuronal processes. The abundant expression of T alpha 1-tubulin mRNA may therefore reflect either a means to increase the available pool of alpha-tubulin or a specific requirement for the T alpha 1 isotype for neurite extension.

subject areas

  • Cell Differentiation
  • Cerebral Cortex
  • Gene Expression Regulation
  • Nervous System
  • Neurons
  • Nucleic Acid Hybridization
  • RNA, Messenger
  • Tissue Distribution
  • Tubulin
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Identity

International Standard Serial Number (ISSN)

  • 0021-9525

Digital Object Identifier (DOI)

  • 10.1083/jcb.105.6.3065

PubMed ID

  • 3693406
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Additional Document Info

start page

  • 3065

end page

  • 3073

volume

  • 105

issue

  • 6

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